MEROSIN, A PROTEIN-SPECIFIC FOR BASEMENT-MEMBRANES OF SCHWANN-CELLS, STRIATED-MUSCLE, AND TROPHOBLAST, IS EXPRESSED LATE IN NERVE AND MUSCLE DEVELOPMENT
MEROSIN, A PROTEIN-SPECIFIC FOR BASEMENT-MEMBRANES OF SCHWANN-CELLS, STRIATED-MUSCLE, AND TROPHOBLAST, IS EXPRESSED LATE IN NERVE AND MUSCLE DEVELOPMENT
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DOI：
10.1073/pnas.85.5.1544
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发表时间：
1988-03-01
影响因子：
11.1
通讯作者：
ENGVALL, E
中科院分区：
文献类型：
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作者：
LEIVO, I;ENGVALL, E
我们利用针对人胎盘蛋白质组分制备的单抗鉴定了一种组织特异性基底膜相关蛋白。免疫荧光染色显示雪旺细胞、横纹肌和滋养层细胞的基底膜呈阳性反应，而与其他基底膜和组织结构均未见反应。在人胎盘蛋白水解物的抗体亲和层析中，这些单抗结合了一个65 kDa的多肽。兔抗血清和针对分离的65 kDa多肽的单抗与原始单抗相同地染色人和猴子组织，并在未经蛋白降解的组织提取液中与80 kDa多肽反应。65 kDa和80 kDa的多肽在免疫学上与层粘连蛋白、IV型胶原、纤维连接蛋白和主要血清蛋白不同。它们可能代表了一种新的基底膜相关蛋白，我们将其命名为Merosin。在已建立的28个细胞系的培养中，均未检测到Merosin免疫反应。在发育中的小鼠组织中，Merosin染色最早出现在新生阶段。Merosin有限的组织分布和发育迟缓的外观表明，该蛋白具有与高水平分化相关的组织特异性功能。
We have identified a tissue-specific basement membrane-associated protein by using monoclonal antibodies prepared against a protein fraction of human placenta. In immunofluorescence, the monoclonal antibodies stained basement membranes of Schwann cells, striated muscle, and trophoblast, whereas no reaction was seen with any other basement membrane or tissue structure. In antibody-affinity chromatography of proteolytic digests of human placenta, a 65-kDa polypeptide was bound by these monoclonal antibodies. Rabbit antisera and monoclonal antibodies raised against the isolated 65-kDa polypeptide stained human and monkey tissues identically to the original monoclonal antibodies and reacted with an 80-kDa polypeptide in tissue extracts prepared without proteolysis. The 65-kDa and 80-kDa polypeptides were shown to be immunologically distinct from laminin, type IV collagen, fibronectin, and major serum proteins. They presumably represent a novel basement membrane-associated protein, which we have named merosin. No merosin immunoreactivity could be detected in cultures of any of 28 established cell lines. In developing mouse tissues, merosin staining first appeared at the newborn stage. The restricted tissue distribution and late developmental appearance of merosin suggest that the protein has a tissue-specific function associated with a high level of differentiation.