Determination of Benzo(a)Pyrene Residue in Sesame Oil by HPLC
Determination of Benzo(a)Pyrene Residue in Sesame Oil by HPLC
复制标题
高效液相色谱法测定芝麻油中苯并(a)芘残留量
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
X. Xi
中科院分区：
文献类型：
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作者：
X. Xi
建立了高效液相色谱法测定芝麻油中苯并（a）芘残留量的方法，样品用正己烷溶解，中性氧化铝柱吸附，苯并（a）芘在0.1 ~ 20 μg/L范围内与峰面积呈良好的线性关系，线性方程为y= 0.0000000，r = 0.9999芝麻油中苯并（a）芘的回收率为73.2%~ 93.2%，相对标准偏差（n=5）为2.2%~ 4.2%，检出限为0.5 μg/kg，方法准确、稳定，适用于芝麻油中苯并（a）芘的测定。
A high performance liquid chromatography(HPLC) method was established for the determination of benzo(a)pyrene residue in sesame oil.Samples were dissolved in hexane,adsorbed on neutral alumina columns,eluted with hexane to remove benzo(a)pyrene and determined by HPLC using fluorescence detector.The linear relationship between peak area and concentration of benzo(a)pyrene were obtained at the range of 0.1-20 μg/L.The linear equation was y=21.226x-0.7335(R2=0.9998);The recovery tests of sesame oil ranged from 73.2% to 93.2%,and RSD(n=5) value were from 2.2% to 4.2%.The detection limit was 0.5 μg/kg.The method was accurate and stable,and is suitable for determining benzo(a) pyrene in the sesame oil.