Serological evidence of infectious salmon anaemia virus (ISAV) infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay (ELISA).
Serological evidence of infectious salmon anaemia virus (ISAV) infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay (ELISA).
复制标题
使用间接酶联免疫吸附测定 (ELISA) 获得养殖鱼类传染性鲑鱼贫血病毒 (ISAV) 感染的血清学证据。
DOI：
10.3354/dao051001
复制
发表时间：
2002
影响因子：
1.4
通讯作者：
F. Kibenge
中科院分区：
文献类型：
--
作者：
M. Kibenge;B. Opazo;A. Rojas;F. Kibenge
抗体检测试验很少用于鱼病的诊断目的。传染性鲑贫血症（Infectious salmon anaemia，ISA）是由传染性鲑贫血病毒（ISAV）引起的大西洋鲑（Atlantic salmon萨尔莫salar L.）该病毒还从智利患病的银鲑Oncorhynchus kisutch中分离出来。间接酶联免疫吸附试验（ELISA），应有利于ISAV感染的血清学诊断，流行病学研究，和ISA在养殖鱼类的控制已开发使用纯化的ISAV作为包被抗原，和单克隆抗体，检测鱼免疫球蛋白结合到板上的抗原。应用该试验对来自加拿大新不伦瑞克芬迪湾的养殖大西洋鲑鱼的随机样品进行检测，阳性鉴定了7条ISAV RT-PCR阳性鱼中的5条，并且所有10条RT-PCR阴性鱼在ELISA中也为阴性。一些RT-PCR阴性的鱼有一个升高的非特异性抗体反应性提示慢性感染或抗ISAV。该测试还能够检测14 coho鲑鱼合并血清样品中的11个来自智利的临床受影响的农场，这些样品通过病毒中和（VN）测试呈阳性，以及4个VN阴性样品中的2个。我们的结论是，这种ELISA将是合适的作为一个常规测试ISAV感染或评估ISAV疫苗的效力之前，将小鲑鱼在海笼，并在海笼中测试鱼类检测ISA的抗性水平。该测定能够与种群减少控制方法组合进行疫苗接种。
Antibody detection tests are rarely used for diagnostic purposes in fish diseases. Infectious salmon anaemia (ISA) caused by ISA virus (ISAV) is an emerging disease of Atlantic salmon Salmo salar L. The virus has also been isolated from diseased coho salmon Oncorhynchus kisutch in Chile. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate serodiagnosis of ISAV infection, the study of epidemiology, and the control of ISA in farmed fishes has been developed using purified ISAV as the coating antigen, and monoclonal antibodies that detect fish immunoglobulins bound to the antigen on the plate. Application of the test to a random sample of farmed Atlantic salmon from the Bay of Fundy, New Brunswick, Canada, positively identified 5 of the 7 ISAV RT-PCR-positive fish, and all 10 RT-PCR-negative fish were also negative in the ELISA. Some RT-PCR-negative fish had an elevated non-specific antibody reactivity suggestive of chronic infection or resistance to ISAV. This test was also able to detect 11 of the 14 coho salmon pooled serum samples from a clinically affected farm in Chile that were positive by the virus neutralization (VN) test, and 2 of the 4 VN-negative samples. We conclude that this ELISA would be suitable as a routine test for ISAV infection or for assessing ISAV vaccine efficacy before placing smolts in sea cages, and for testing fishes in sea cages to detect level of resistance to ISA. The assay enables vaccination in combination with depopulation control methods.
计算机物理通信。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--