Testis morphometry, seminiferous epithelium cycle length, and daily sperm production in domestic cats (Felis catus)
Testis morphometry, seminiferous epithelium cycle length, and daily sperm production in domestic cats (Felis catus)
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DOI：
10.1095/biolreprod.102.010652
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发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Godinho, CL
中科院分区：
文献类型：
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作者：
França, LR;Godinho, CL
关于家猫睾丸结构和功能的信息很少，主要是与生精上皮细胞周期和精子产生有关的数据。本研究中研究的猫睾丸重量为1.2 g。与大多数研究的哺乳动物物种相比，猫的睾丸质量与体重（性腺指数）相关的值为0.08%，非常低。这些动物的图尼卡白蛋白体积密度（%）相对较高，约占睾丸的19%。猫的生精小管和间质细胞体积密度M分别约为90%和6%。曲细精管的平均直径为220 μ m，每克睾丸中的曲细精管直径为23 μ m。根据管状形态系统表征的周期的八个阶段的频率如下：阶段1，24.9%;阶段2，12.9%;阶段3，7.7%;阶段4，17.6%;阶段5，7.2%;阶段6，11.9%;阶段7，6.8%;和阶段8，11%。减数分裂前和减数分裂后阶段的频率分别为46%和37%。生精上皮每个周期的持续时间为10.4天，按4.5个周期计算，精子发生的总持续时间为46.8天。每个粗线期初级精母细胞的圆形精子细胞数（减数分裂指数）为2.8，这意味着在两次减数分裂期间发生了显著的细胞损失（30%）。在第1周期，每个支持细胞核仁中的生殖细胞总数和圆形精子细胞数分别为9.8和5.1。Leydig细胞体积约为2000 mum（3），细胞核体积为260 mum（3）。每克猫睾丸的Leydig和Sertoli细胞数量约为3000万。猫每克睾丸的每日精子产量（精子发生效率）约为1600万。据我们所知，这是首次对家猫睾丸结构和功能进行更详细和全面的研究。此外，这是文献中第一次报告显示每克睾丸的支持细胞和间质细胞数量以及任何种类猫科动物的每日精子产量。在这方面，除了提供与其他猫科动物进行比较研究的背景外，本工作中获得的数据可能有助于未来的研究，其中家猫可以作为适当的受体模型，用于使用生殖细胞移植技术保存稀有或濒危野生猫科动物的遗传资源。
There is very little information regarding the testis structure and function in domestic cats, mainly data related to the cycle of seminiferous epithelium and sperm production. The testis weight in cats investigated in the present study was 1.2 g. Compared with most mammalian species investigated, the value of 0.08% found for testes mass related to the body mass (gonadosomatic index) in cats is very low. The tunica albuginea volume density (%) in these animals was relatively high and comprised about 19% of the testis. Seminiferous tubule and Leydig cell volume density M in cats were approximately 90% and 6%, respectively. The mean tubular diameter was 220 mum, and 23 m of seminiferous tubule were found per testis and per gram of testis. The frequencies of the eight stages of the cycle, characterized according to the tubular morphology system, were as follows: stage 1, 24.9%; stage 2, 12.9%; stage 3, 7.7%; stage 4, 17.6%; stage 5, 7.2%; stage 6, 11.9%; stage 7, 6.8%; and stage 8, 11 %. The premeiotic and postmeiotic stage frequency was 46% and 37%, respectively. The duration of each cycle of seminiferous epithelium was 10.4 days and the total duration of spermatogenesis based on 4.5 cycles was 46.8 days. The number of round spermatids for each pachytene primary spermatocytes (meiotic index) was 2.8, meaning that significant cell loss (30%) occurred during the two meiotic divisions. The total number of germ cells and the number of round spermatids per each Sertoli cell nucleolus at stage 1 of the cycle were 9.8 and 5.1, respectively. The Leydig cell volume was approximately 2000 mum(3) and the nucleus volume 260 mum(3). Both Leydig and Sertoli cell numbers per gram of testis in cats were approximately 30 million. The daily sperm production per gram of testis in cats (efficiency of spermatogenesis) was approximately 16 million. To our knowledge, this is the first investigation to perform a more detailed and comprehensive study of the testis structure and function in domestic cats. Also, this is the first report in the literature showing Sertoli and Leydig cell number per gram of testis and the daily sperm production in any kind of feline species. in this regard, besides providing a background for comparative studies with other felids, the data obtained in the present work might be useful in future studies in which the domestic cat could be utilized as an appropriate receptor model for preservation of genetic stock from rare or endangered wild felines using the germ cell transplantation technique.