A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes.
A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes.
复制标题
用于人类卵母细胞运输特性的芯片表征的微流体灌注方法
DOI：
10.1039/c6lc01532h
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发表时间：
2017-03-29
期刊：
影响因子：
6.1
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Zhao G;Zhang Z;Zhang Y;Chen Z;Niu D;Cao Y;He X
准确表征人类卵母细胞的细胞膜转运特性对于生殖药理学、生育力保存和辅助生殖具有重要意义。然而，用于量化传输属性的常用手动方法与不受控制的操作员之间和运行之间的变异性相关。在这里，我们报告了一种新型夹心结构微流体装置，可以轻松制造用于表征卵母细胞膜运输特性。由于其对微通道中溶液置换和温度的良好控制能力，可以精确表征卵母细胞膜的温度依赖性通透性。此外，灌注后的受精和发育能力分析表明我们的方法不会损害体外成熟人类卵母细胞的生理功能。总的来说，我们提出了一种基于新型三明治结构微流体装置的方法的开发，该方法允许在无害渗透压休克或细胞损伤下对人类卵母细胞的运输特性进行芯片表征。
Accurate characterization of the cell membrane transport properties of human oocytes is of great significance to reproductive pharmacology, fertility preservation, and assisted reproduction. However, the commonly used manual method for quantifying the transport properties is associated with uncontrolled operator-to-operator and run-to-run variability. Here, we report a novel sandwich structured microfluidic device that can be readily fabricated for characterizing oocyte membrane transport properties. Owing to its capacity of excellent control of both solution replacement and temperature in the microchannel, the temperature-dependent permeability of oocyte membrane can be precisely characterized. Furthermore, the fertilization and developmental competence analysis post perfusion indicate that our approach does not compromise the physiological function of in-vitro matured human oocytes. Collectively, we present the development of a novel sandwich structured microfluidic device based approach that allows for on-chip characterization of the transport properties of human oocytes under innocuous osmotic shock or injury to the cells.