Laboratory evaluation of a potential diesel fuel interference in the determination of polycyclic aromatic compounds on dermal samplers.
Laboratory evaluation of a potential diesel fuel interference in the determination of polycyclic aromatic compounds on dermal samplers.
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实验室评估柴油燃料对皮肤采样器测定多环芳香族化合物的潜在干扰。
DOI：
10.1080/15459620490445444
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发表时间：
2004
影响因子：
2
通讯作者：
Rinehart,RichardD
中科院分区：
文献类型：
--
作者：
Weker,RobertA;Herrick,RobertF;Rinehart,RichardD
道路摊铺机通过皮肤接触、吸入或摄入而暴露于沥青烟雾。这些烟雾含有多环芳香族化合物 (PAC)，这是一类包括多环芳香烃 (PAH) 的化合物。 PAH 包括芘和苯并(a)芘等化合物以及大量已知或疑似致癌的其他化合物。为了估计沥青烟雾颗粒物质的皮肤沉积，可以将聚丙烯采样贴片佩戴在工人的手腕内侧。用于清洁的柴油可能会溅到皮肤采样器上。对暴露的真皮贴片进行溶剂萃取，并使用 NIOSH 方法 5800 的修改版估算提取物中的 PAC。最终溶液通过高压液相色谱 (HPLC) 和荧光检测器（使用 270 纳米的激发波长和 415 纳米的发射波长）进行分析。 PAC 浓缩在 C-18 反相保护柱上，但不分离，以便可以方便地作为一组进行测量。在本研究中，我们检查了沉积的柴油是否会干扰 PAC 的 HPLC 测定。我们描述了柴油污染对广泛的分析物和干扰物的影响。皮肤采样器上滴两滴（约 100 µL）柴油会导致多环芳香族化合物 (PAC) 的浓度被高估。在我们现场研究确定的 PAC 范围内（每个样品 0 至 54 μg），这些误差可能超过 100%。由于 100 µL 沉积的柴油产生了清晰的视觉“湿度”，因此该观察结果可以作为样品剔除的标准之一。必须仔细检查采样介质是否存在可见污染，以防止沥青烟沉积的 PAC 测量出现偏差。
Road pavers are exposed to asphalt fumes through dermal contact, inhalation, or ingestion. These fumes contain polycyclic aromatic compounds (PACs), a class of compounds including polycyclic aromatic hydrocarbons (PAHs). The PAHs include compounds such as pyrene and benzo(a)pyrene and a large number of other compounds of known or suspected carcinogenicity. To estimate dermal deposition of asphalt fume particulate matter, polypropylene sampling patches can be worn on a worker's inside wrist. Diesel fuel used for cleaning can splash onto the dermal sampler. Exposed dermal patches were solvent extracted and PACs estimated in the extract using a modification of NIOSH Method 5800. The final solution was analyzed by high pressure liquid chromatography (HPLC) with a fluorescence detector using an excitation wavelength of 270 nanometers and an emission wavelength of 415 nanometers. The PACs were concentrated on a C-18 reverse phase guard column but not separated so that they could be measured expediently as a group. In this study we examined whether deposited diesel fuel interferes with the HPLC determination of PACs. We characterized the effect of diesel fuel contamination over broad ranges of both analyte and interferent. Two drops, or approximately 100 μL, of diesel fuel on the dermal samplers caused an overestimation of the concentration of polycyclic aromatic compounds (PACs). In the range of PACs determined in our field study (0 to 54 μg per sample), these errors could have exceeded 100%. Because 100 μL of deposited diesel fuel produced a clear visual “wetness,” this observation can serve as one of the criteria for sample rejection. Sampling media must be examined carefully for visible contamination to prevent bias in the measurement of PACs deposited from asphalt fume.