A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis.
A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis.
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DOI：
10.1016/j.biortech.2021.126275
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发表时间：
2021-11
影响因子：
11.4
通讯作者：
Litao Zhang;Chunhui Zhang;R. Xu;WEN-JIE Yu;Jianguo Liu
中科院分区：
文献类型：
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作者：
Litao Zhang;Chunhui Zhang;R. Xu;WEN-JIE Yu;Jianguo Liu
本研究探讨了雨生红球藻（Haematococcus pluvialis）交替氧化酶（AOX）呼吸途径抑制对脂肪酸和虾青素生物合成相关过程的促进作用机制。AOX途径的限制诱导了脂肪酸和虾青素合成所需的活性氧、NAD（P）H及其底物（乙酰辅酶A、丙酮酸和甘油醛-3-磷酸）的积累，从而促进了碳通量进入脂肪酸和虾青素生物合成。在9天的培养期内，当AOX途径被抑制约37.7%时，脂肪酸和虾青素含量分别增加了20.6%和20.7%。在大规模培养的条件下，去除氮磷营养盐、添加抑制剂和调节空气/CO2通气量可以抑制AOX途径。雨生植物因此，本研究提供了一个有用的增强策略，脂肪酸和虾青素的共同生产和阐明的AOX途径在调节脂肪酸和虾青素的生物合成的作用。
In the present study, the mechanisms for facilitating fatty acid and astaxanthin biosynthesis-related processes by inhibiting the alternative oxidase (AOX) respiratory pathway inHaematococcus pluvialiswas investigated. The restriction of the AOX pathway induced the accumulation of reactive oxygen species, NAD(P)H and its substrates (acetyl-CoA, pyruvate and glyceraldehy-3-phosphate), which are required for fatty acid and astaxanthin production, thereby promoting the carbon flux into fatty acid and astaxanthin biosynthesis. During a 9-day incubation period, the fatty acid and astaxanthin contents increased by 20.6% and 20.7%, respectively, when the AOX pathway was inhibited approximately 37.7%. The AOX pathway may be inhibited by nutrient (nitrogen and phosphorus) removal, inhibitor addition and air/CO2aeration adjustments in the large-scale cultivation ofH. pluvialis. Therefore, the current study provides a useful enhancement strategy for fatty acid and astaxanthin coproduction and elucidates the roles of the AOX pathway in regulating fatty acid and astaxanthin biosynthesis.