Increase of insulin sensitivity by stevioside in fructose-rich chow-fed rats
Increase of insulin sensitivity by stevioside in fructose-rich chow-fed rats
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DOI：
10.1055/s-2005-870528
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发表时间：
2005-10-01
影响因子：
2.2
通讯作者：
Cheng, JT
中科院分区：
文献类型：
--
作者：
Chang, JC;Wu, MC;Cheng, JT
近年来，世界各地，特别是发达国家，膳食果糖的摄入量显著增加。甜菊苷是菊科植物甜叶菊（Stevia rebaudiana Bertoni）叶中的一种糖苷，用于筛选含60%果糖的饮食对大鼠胰岛素抵抗的影响。单次口服甜菊苷90分钟，以剂量依赖性方式降低接受富含果糖食物四周的大鼠的血糖浓度。此外，使用葡萄糖-胰岛素指数（葡萄糖曲线下面积的乘积）测量胰岛素对葡萄糖处置率的作用，并在腹膜内葡萄糖耐量试验期间测量胰岛素。在给予4周富含果糖的食物90分钟的大鼠中口服给予甜菊苷（5.0 mg/kg）逆转了葡萄糖-胰岛素指数的值，表明甜菊苷具有改善该胰岛素抵抗动物模型中的胰岛素敏感性的能力。对甲苯磺丁脲（10.0 mg/kg，i. p.）在富含果糖的食物喂养的大鼠中，与载体治疗组相比，每天三次重复的甜菊苷治疗也显著延迟。甲苯磺丁脲的血浆葡萄糖降低活性被引入以解释内源性胰岛素分泌的不同水平，并且被广泛用作胰岛素抵抗发展的指标。因此，它提供了支持性的数据，反复口服甜菊苷延缓了高果糖饮食大鼠胰岛素抵抗的发展。在链脲佐菌素诱导的糖尿病大鼠（STZ-糖尿病大鼠）中，使用外源性胰岛素的血浆葡萄糖降低作用进一步鉴定了通过甜菊苷给药增加的胰岛素敏感性。STZ糖尿病大鼠口服0.2 mg/kg甜菊苷，每日三次，持续10天，增加了对外源性胰岛素的反应。综上所述，这表明口服给予甜菊苷改善了胰岛素敏感性，并且似乎适合作为糖尿病患者和/或消耗大量果糖的患者的佐剂。
The intake of dietary fructose has undergone a marked increase around the world, especially the developed countries, in recent times. Stevioside, a glycoside contained in the leaves of Stevia rebaudiana Bertoni (Compositae), was used to screen the effect induced by a diet containing 60% fructose on insulin resistance in rats. Single oral administration of stevioside for 90 min decreased plasma glucose concentrations in a dose-dependent manner in rats receiving fructose-rich chow for four weeks. In addition, insulin action on glucose disposal rate was measured using the glucose-insulin index, the product of the areas under the curve of glucose, and insulin during the intraperitoneal glucose tolerance test. Oral administration of stevioside (5.0 mg/kg) in rats given four weeks of fructose-rich chow for 90 min reversed the value of glucose-insulin index, indicating that stevioside has the ability to improve insulin sensitivity in this insulin-resistant animal model. Time for the loss of plasma glucose lowering response to tolbutamide (10.0 mg/kg, i.p.) in fructose-rich chow fed rats was also markedly delayed by repeated stevioside treatment three times daily compared to the vehicle-treated group. The plasma glucose-lowering activity of tolbutamide was introduced to account for varying levels of endogenous insulin secretion, and is widely used as the indicator of insulin resistance development. Thus, it provided the supportive data that repeated oral administration of stevioside delayed the development of insulin resistance in rats on a high-fructose diet. Increased insulin sensitivity by stevioside administration was further identified using the plasma glucose-lowering action of exogenous insulin in streptozotocin-induced diabetic rats (STZ-diabetic rats). Oral administration of stevioside at 0.2 mg/kg three times daily into STZ-diabetic rats for ten days increased the response to exogenous insulin. Taken together, this demonstrated that oral administration of stevioside improves insulin sensitivity, and seems suitable as an adjuvant for diabetic patients and/ or those that consume large amounts of fructose.