Effects of coffee consumption on iron, zinc and copper status in nonpregnant and pregnant Sprague-Dawley rats
Effects of coffee consumption on iron, zinc and copper status in nonpregnant and pregnant Sprague-Dawley rats
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DOI：
10.3109/09637489709012591
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发表时间：
1997-05-01
影响因子：
3.9
通讯作者：
Dewey, KG
中科院分区：
文献类型：
--
作者：
Aldrian, PS;Keen, CL;Dewey, KG
为了研究咖啡摄入对铁、锌和铜状态的影响，64只雌性sd大鼠被分为四组：未怀孕的咖啡、未怀孕的水、怀孕的咖啡和怀孕的水。咖啡组用冻干咖啡溶液（1.1% w/v）作为鞋底。18天研究期间的液体来源；所有组均饲喂边际含铁自由AD的纯化饲粮。在妊娠第18天，大鼠空腹8 h后插管0.033 MBq Fe-59，灌胃后4、8、12、24 h处死。咖啡组的液体摄入量明显较低。咖啡对怀孕或未怀孕大鼠的食物摄入、体重增加、血红蛋白或红细胞压积都没有影响。在未怀孕的大鼠中，咖啡组的Fe-59在血浆中的百分比低于对照组，在脾脏和红细胞中的百分比高于对照组；在怀孕大鼠中，咖啡组的胃（仅在4小时内）、血液、脾脏和骨骼中的Fe-59比例高于对照组。在未怀孕组中，咖啡对铁、锌和铜的组织浓度没有显著影响，但喝了咖啡的怀孕大鼠肝脏中铁的浓度很高；骨骼和胎盘，胎盘锌浓度较低。与对照组相比，咖啡组胎儿的体重、身高和铁-59百分比都有所下降，但肝脏铁含量较高。结果表明，咖啡摄入导致(a)红细胞生成增加，这是铁缺乏的一种迹象，在未怀孕和怀孕的大鼠中，以及(b)胎盘铁运输受损。
To investigate the influence of coffee consumption on iron, zinc and copper status, 64 Sprague-Dawley female rats were assigned to four treatment groups: nonpregnant given coffee, nonpregnant-given water, pregnant-given coffee and pregnant-given water. The coffee groups received a freeze-dried coffee solution (1.1% w/v) as the sole. source of liquids during the 18-day study period; all groups were provided a purified diet marginal in iron ad libitum. On day 18 (of gestation in the pregnant rats), rats were intubated with 0.033 MBq Fe-59 after an 8 h fast and killed 4, 8, 12 or 24 h post-gavage. Fluid intake was significantly lower in the coffee groups. Coffee did not affect food consumption, weight gain, hemoglobin or hematocrit in-either pregnant or nonpregnant rats. In nonpregnant rats, the percentage of Fe-59 was lower in plasma and higher in the spleen and erythrocytes in the coffee group than in controls; in pregnant rats the coffee group had a higher percentage of Fe-59 in the stomach (at 4 h only), blood, spleen and bone than controls. There were no significant effects of coffee on tissue concentrations of Fe, Zn or Cu in the nonpregnant group, but pregnant rats given coffee had high concentrations,of Fe in the liver; bone and placenta, and low placenta Zn concentrations compared to controls. In fetuses, there were trends towards lower weight, length, and percentage Fe-59, but higher liver Fe, in the coffee group than rin the controls. The results suggest that coffee intake leads to (a) increased attempts at erythropoeisis, an indication of iron deficiency, in both nonpregnant and pregnant rats, and (b) impaired placental Fe transport.