Effects of nanosecond pulsed electric fields application on cancer cell and combination of anticancer drug
Effects of nanosecond pulsed electric fields application on cancer cell and combination of anticancer drug
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DOI：
10.1002/eej.23376
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发表时间：
2022-03-02
影响因子：
0.4
通讯作者：
Shimomura, Naoyuki
中科院分区：
文献类型：
--
作者：
Enomoto, Soichiro;Konishi, Daisuke;Shimomura, Naoyuki
为了建立一种新的癌症治疗方法，利用纳秒脉冲电场（nsPEFs），我们研究了nsPEFs和抗癌药物（阿霉素，ADM）对癌细胞的影响。与对照相比，nsPEFs应用显著降低了癌细胞的存活分数。与单独的nsPEFs和单独的ADM给药相比，在nsPEFs应用和ADM给药的组合中观察到存活分数的显著降低。此外，nsPEFs应用和ADM施用的存活分数的乘数值被计算为伪组合效应。nsPEFs与ADM联合的实验结果的存活分数低于假联合的结果。因此，证实了nsPEFs和ADM组合的协同效应。此外，nsPEFs应用后ADM给药的存活分数显著低于nsPEFs应用前24 h ADM给药的存活分数。由于应用nsPEFs形成纳米孔，ADM对细胞的作用将是有效的。流式细胞仪检测细胞凋亡和坏死实验证实ADM和nsPEF可诱导细胞凋亡。
With the goal of establishing a new cancer treatment method using nanosecond pulsed electric fields (nsPEFs), we investigated the effect of applying nsPEFs and anticancer drugs (adriamycin, ADM) to cancer cells. The surviving fraction of cancer cells was significantly reduced by nsPEFs application compared to the control. Compared to nsPEFs alone and ADM administration alone, a significant decrease of the surviving fraction was observed in combination of nsPEFs application and ADM administration. In addition, multiplying value of surviving fractions of nsPEFs application and ADM administration was calculated as a pseudo-combination effect. The surviving fractions of the experimental combinational results of nsPEFs and ADM were lower than the pseudo-combination results. Therefore, the synergistic effect of combination of nsPEFs and ADM was confirmed. Furthermore, the surviving fraction of ADM administration after nsPEFs application was significantly lower than that of ADM administration on 24 h before nsPEFs application. ADM action on cells would be effective owing to nanopores were formed by applying nsPEFs. It was confirmed that ADM and nsPEF induced apoptosis of cells from apoptosis and necrosis test using FACS.