Biological Properties of Dialysable Peptides Derived from Plasmin Digestion of Bovine Fibrinogen Preparations
Biological Properties of Dialysable Peptides Derived from Plasmin Digestion of Bovine Fibrinogen Preparations
复制标题
纤溶酶消化牛纤维蛋白原制剂所得可透析肽的生物学特性
DOI：
10.1055/s-0038-1647964
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发表时间：
1976
影响因子：
6.7
通讯作者：
M. Donati
中科院分区：
文献类型：
--
作者：
W. Buczko;G. Gaetano;R. Franco;M. Donati
摘要可透析肽（M. W.少于12，000）通过纯化的牛纤维蛋白原的纤溶酶消化获得。在三种不同的测试系统中研究了这些肽在大鼠中的生物学效应：ADP诱导的血小板聚集、离体心房收缩性和血管通透性。由肽诱导的作用是：抑制ADP诱导的血小板聚集，增加离体心房收缩的频率和局部血管通透性增加。所有这些活动都是浓度依赖性的。6微克的肽仍然有效地增加血管通透性;在体外系统中，最小有效剂量范围为165 μ g/ml至650 μg/ml。用重蒸馏水通过Sephadex G-25凝胶洗脱后，得到四个级分。第二部分（M.W.约5，000）是最活跃的，其次是第一个，然后是第三个;第四个部分是无活性的。这些数据表明，肽在体内的局部积累可能具有临床相关性。
Summary Dialysable peptides (M. W. less than 12,000) were obtained by plasmin digests of purified bovine fibrinogen. The biological effects of these peptides were studied in rats in three different test systems: ADP-induced platelet aggregation, isolated atria contractility and vascular permeability. The effects induced by the peptides were: inhibition of ADP-induced platelet aggregation, increase in the frequency of isolated atria contractions and local increase in vascular permeability. All these activities were concentration dependent. Six micrograms of the peptides were still effective in increasing vascular permeability; in the in vitro systems the smallest effective dose ranged between 165 and 650 μg/ml. Following elution through a Sephadex G-25 gel with bidistilled water, four fractions were obtained. The second fraction (M.W. about 5,000) was the most active, followed by the first and then the third one; the fourth fraction was inactive. These data suggest that local accumulation of peptides in vivo may be of clinical relevancy.