Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue
Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue
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DOI：
10.1006/excr.1999.4440
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发表时间：
1999-05-01
影响因子：
3.7
通讯作者：
Abrams, JM
中科院分区：
文献类型：
--
作者：
Grinnell, F;Zhu, MF;Abrams, JM
在体外肉芽组织模型中，机械张力作用下的早期传代人二倍体成纤维细胞很少或没有凋亡。机械张力的释放引发细胞凋亡反应，凋亡反应在3 ~ 6 h内发生，24 h达到平稳期。凋亡细胞百分比（约15%）保持不变，持续7 d， 3 d后细胞总数下降。在不应用药物或遗传干预的情况下，确定机械卸载作为细胞凋亡的刺激，是一种新的观察结果，使我们能够模拟伤口愈合过程中发生的类似事件。对这些条件下调节细胞凋亡机制的研究表明，细胞凋亡反应不需要细胞分化成肌成纤维细胞，而是由胶原基质中的机械张力和生长因子共同控制的。(C) 1999学术出版社。
In an in vitro model of granulation tissue, early passage human diploid fibroblasts under mechanical tension showed little or no apoptosis. Release of mechanical tension triggered an apoptotic response that occurred within 3-6 h and reached a plateau by 24 h. The percentage of apoptotic cells (similar to 15%) remained constant up to 7 days, and after 3 days, total cell number declined. Identification of mechanical unloading as a stimulus for apoptosis, without application of pharmacologic or genetic intervention, is a novel observation that permits us to model similar events that occur during wound healing. Studies on the mechanism regulating apoptosis under these conditions established that the apoptotic response does not require differentiation of cells into myofibroblasts but is governed by a combination of mechanical tension and growth factors in the collagen matrix. (C) 1999 Academic Press.