Isolation and characterization of the lipopolysaccharide of Thiocapsa roseopersicina.
Isolation and characterization of the lipopolysaccharide of Thiocapsa roseopersicina.
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Thiocapsa Rosepersicina 脂多糖的分离和表征。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
H. Mayer
中科院分区：
文献类型：
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作者：
R. E. Hurlbert;J. Weckesser;R. Tharanathan;H. Mayer
用苯酚/水比色法从水相中分离出玫瑰硫囊藻脂多糖。用10%的冰醋酸(100℃，3小时)将其裂解成多糖部分(降解的多糖)和脂类A。D-甘露糖、L-鼠李糖、3-氨基-3，6-二脱氧-D-半乳糖和D-葡萄糖是降解多糖的主要成分。2-O-甲基-L-鼠李糖、3-O-甲基-D-甘露糖、D-半乳糖、氨基葡萄糖和喹诺酮胺为次要成分。仅检出少量D-甘露-D-甘露-庚糖和3-脱氧-D-甘露-辛酸。值得注意的是，玫瑰红曲霉的类脂A除了含有D-氨基葡萄糖外，还含有一种中性糖--D-甘露糖，就像从花色菌D中观察到的类脂A一样。主要脂肪酸是β-羟基对称脂肪酸和月桂酸。只有微量的磷被发现，这表明该脂A是不含磷的。玫瑰毛滴虫的脂多糖代表该菌株的O-抗原。它与被动血凝中针对活细胞或热灭活细胞制备的抗血清发生反应。
The lipopolysaccharide from Thiocapsa roseopersicina was isolated by phenol/water, being found in the water phase. It is cleaved into a polysaccharide moiety (degraded polysaccharide) and lipid A by hydrolysis with 10% acetic acid (100 degree C, 3 h). D-Mannose, L-rhamnose, 3-amino-3, 6-dideoxy-D-galactose and D-glucose are the major constituents of the degraded polysaccharide. 2-O-Methyl-L-rhamnose, 3-O-methyl-D-mannose, D-galactose, glucosamine and quinovosamine are minor constituents. D-Glycer-D-manno-heptose (tentatively identified) and 3-deoxy-D-manno-octulosonic acid were detected in only small amounts. Conspicuously, lipid A from T. roseopersicina contains a neutral sugar, D-mannose, in addition to D-glucosamine, as had been observed with lipid A from Chromatium vinosum D. Major fatty acids are beta-hydroxymyristic and lauric acids. Only trace amounts of phosphorus were found indicating this lipid A to be free of phosphate. The lipopolysaccharide of T. roseopersicina represents the O-antigen of the strain. It reacts with antisera prepared against living or heat-killed cells in passive hemagglutination.