Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica)
Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica)
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普马拉汉坦病毒感染（流行性肾病）诊断血清学方法评价
DOI：
10.1128/jcm.35.12.3264-3268.1997
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发表时间：
1997
影响因子：
9.4
通讯作者：
Å. Lundkvist
中科院分区：
文献类型：
--
作者：
K. B. Sjölander;F. Elgh;H. Kallio;O. Vapalahti;Madeleine Hägglund;Viktoria Palmcrantz;P. Juto;A. Vaheri;B. Niklasson;Å. Lundkvist
流行性肾病(NE)，即Puumala(PUU)病毒感染，是一种常见的与急性肾功能损害相关的热性疾病。为了将NE与其他急性发热性疾病区分开来，快速和可靠的血清学诊断是重要的，最近已经引入了一些不同的方案。在本报告中，我们描述了基于天然的、大肠杆菌表达的或杆状病毒表达的核衣壳蛋白(N)的6种PUU病毒免疫球蛋白(IgM)和7种Ig G酶联免疫吸附试验(ELISA)的比较评价。中和法和免疫荧光法用于比较。用三种基于MU捕捉法的IgM ELISA，分别使用天然的、杆状病毒表达的和大肠杆菌表达的N抗原，以及基于纯化的大肠杆菌表达的全长N吸附到固相上的ELISA，都获得了同样高的敏感性和特异性。基于截短的氨基末端N蛋白的检测，包括商业上可用的PUU病毒IgM ELISA，都显示出较低的敏感性。对于PUU病毒特异性免疫球蛋白的检测，基于单抗捕获的天然或杆状病毒表达的N抗原的ELISA具有最好的敏感性和特异性，而基于大肠杆菌表达的N的检测方法并不能检测所有PUU病毒抗体阳性的血清样本。一种基于大肠杆菌表达的氨基末端N的商品化PUU病毒Ig G酶联免疫吸附试验的敏感性显著低于其他所有的Ig G检测方法。
Nephropathia epidemica (NE), Puumala (PUU) virus infection, is a febrile disease which is commonly associated with acute renal impairment. To differentiate NE from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. In the present report we describe a comparative evaluation of six PUU virus immunoglobulin M (IgM) and seven IgG enzyme-linked immunosorbent assay (ELISA) protocols based on native, Escherichia coli-expressed, or baculovirus-expressed nucleocapsid protein (N). Neutralization and immunofluorescence assays were included for comparison. Equally high sensitivities and specificities were obtained with three mu-capture-based IgM ELISAs using native, baculovirus-expressed, and E. coli-expressed N antigens, respectively, and by an ELISA based on purified E. coli-expressed full-length N adsorbed to solid phase. The assays based on truncated amino-terminal N proteins, including a commercially available PUU virus IgM ELISA, all showed lower sensitivities. For detection of PUU virus-specific IgG, ELISAs based on monoclonal antibody-captured native or baculovirus-expressed N antigens showed optimal sensitivities and specificities, while the assays based on E. coli-expressed N did not detect all PUU virus IgG-positive serum samples. A commercially available PUU virus IgG ELISA based on E. coli-expressed amino-terminal N showed a significantly lower sensitivity than those of all other IgG assays.