Detecting Lyme disease using antibody-functionalized single-walled carbon nanotube transistors.
Detecting Lyme disease using antibody-functionalized single-walled carbon nanotube transistors.
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DOI：
10.1016/j.bios.2013.01.035
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发表时间：
2013-07-15
影响因子：
12.6
通讯作者：
Johnson, A. T. Charlie
中科院分区：
文献类型：
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作者：
Lerner, Mitchell B.;Dailey, Jennifer;Goldsmith, Brett R.;Brisson, Dustin;Johnson, A. T. Charlie
We examined the potential of antibody-functionalized single-walled carbon nanotube (SWNT) field-effect transistors (FETs) for use as a fast and accurate sensor for a Lyme disease antigen. Biosensors were fabricated on oxidized silicon wafers using chemical vapor deposition grown carbon nanotubes that were functionalized using diazonium salts. Attachment of Borrelia burgdorferi (Lyme) flagellar antibodies to the nanotubes was verified by Atomic Force Microscopy and electronic measurements. A reproducible shift in the turn-off voltage of the semiconducting SWNT FETs was seen upon incubation with Borrelia burgdorferi flagellar antigen, indicative of the nanotube FET being locally gated by the residues of flagellar protein bound to the antibody. This sensor effectively detected antigen in buffer at concentrations as low as 1 ng/ml, and the response varied strongly over a concentration range coinciding with levels of clinical interest. Generalizable binding chemistry gives this biosensing platform the potential to be expanded to monitor other relevant antigens, enabling a multiple vector sensor for Lyme disease. The speed and sensitivity of this biosensor make it an ideal candidate for development as a medical diagnostic test.
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影响因子：
17.1
作者：
Lerner MB;D'Souza J;Pazina T;Dailey J;Goldsmith BR;Robinson MK;Johnson AT
通讯作者：
Johnson AT
影响因子：
3.3
作者：
Johnson, Robert R.;Rego, Blake Jon;Klein, Michael L.
通讯作者：
Klein, Michael L.
影响因子：
9.4
作者：
Bakken, LL;Callister, SM;Schell, RF
通讯作者：
Schell, RF
影响因子：
56.9
作者：
Strano, MS;Dyke, CA;Smalley, RE
通讯作者：
Smalley, RE
影响因子：
38.3
作者：
通讯作者：
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