Analysis of the proenkephalin second messenger-inducible enhancer in rat striatal cultures.
Analysis of the proenkephalin second messenger-inducible enhancer in rat striatal cultures.
复制标题
大鼠纹状体培养物中脑啡肽原第二信使诱导增强子的分析。
DOI：
10.1046/j.1471-4159.1995.65031007.x
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发表时间：
1995
影响因子：
4.7
通讯作者：
Hyman,SE
中科院分区：
文献类型：
--
作者：
Konradi,C;Cole,RL;Green,D;Senatus,P;Leveque,JC;Pollack,AE;Grossbard,SJ;Hyman,SE
We have previously shown that in cell extracts from rat striatum, cyclic AMP response element (CRE) binding protein (CREB), rather than AP‐1 proteins, preferentially interacts with the CRE‐2 element of the proenkephalin second messenger‐inducible enhancer, even under conditions in which AP‐1 proteins are highly induced. Here we use primary striatal cultures to permit a more detailed analysis of CRE‐2 function and protein binding in relevant neural cell types. By transfection we find that in primary striatal cultures, as in transformed cell lines, the CRE‐1 and CRE‐2 elements are required for significant induction by cyclic AMP. We report that cyclic AMP induction of the proenkephalin gene in striatal cultures is protein synthesis independent, excluding a role for newly synthesized proteins like c‐Fos. We also show that cyclic AMP induces CREB phosphorylation and that phosphorylated CREB interacts strongly with CRE‐2 and weakly with CRE‐1. The predominant protein bound to CRE‐1 is not CREB, however, and remains to be identified. Despite some prior predictions, we do not find a role for c‐Fos in cyclic AMP regulation of proenkephalin gene expression in neurons.
