Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy
Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy
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DOI：
10.1126/science.abb8501
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发表时间：
2020-07-03
期刊：
影响因子：
56.9
通讯作者：
Bassler, Bonnie L.
中科院分区：
文献类型：
--
作者：
Qin, Boyang;Fei, Chenyi;Bassler, Bonnie L.
细菌生物膜代表了多细胞组织的基本形式，赋予组成细胞生存优势。已经在病原体和模型生物膜形成霍乱弧菌中研究了生物膜形成过程中细胞排序的连续阶段。目前尚不清楚单个细胞的空间轨迹和许多细胞的集体运动如何驱动生物膜扩张。我们开发了双视图光片显微镜来研究生物膜从创始细胞到成熟三维群落的发育动态。对单个细胞的追踪揭示了两种不同的命运：一组生物膜细胞向外弹道扩张，而另一组则被困在基底上。集体喷泉般的流动将细胞输送到生物膜前沿，绕过被困在基底上的成员并促进横向生物膜扩张。这种集体流动模式是通过生物膜生长对抗基底摩擦的连续体模型定量捕获的。协调的细胞运动需要基质蛋白 RbmA，没有它细胞就会不稳定地增殖。因此，跟踪空间和时间上的细胞谱系和轨迹揭示了多细胞结构是如何从单个创始细胞形成的。
Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former Vibrio cholerae. It is unknown how spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion. We developed dual-view light-sheet microscopy to investigate the dynamics of biofilm development from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward, while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front, bypassing members trapped at the substrate and facilitating lateral biofilm expansion. This collective flow pattern was quantitatively captured by a continuum model of biofilm growth against substrate friction. Coordinated cell movement required the matrix protein RbmA, without which cells expanded erratically. Thus, tracking cell lineages and trajectories in space and time revealed how multicellular structures form from a single founder cell.