The effects of wound lavage solutions on canine fibroblasts: an in vitro study.
The effects of wound lavage solutions on canine fibroblasts: an in vitro study.
复制标题
伤口灌洗液对犬成纤维细胞的影响：体外研究。
DOI：
10.1111/j.1532-950x.1997.tb00517.x
复制
发表时间：
1997
影响因子：
1.8
通讯作者：
S. Swaim
中科院分区：
文献类型：
--
作者：
E. Buffa;A. Lubbe;F. Verstraete;S. Swaim
目标
本研究的目的是在体外模型中确定磷酸盐缓冲盐水 (PBS)、无菌自来水、生理盐水和林格氏乳酸盐对伤口愈合的影响。
研究设计
确定了 PBS、无菌自来水、生理盐水和林格氏乳酸盐对犬胚胎成纤维细胞原代系的影响。
动物或样本群体
犬胚胎成纤维细胞的初级系。
方法
通过以下方式确定各种灌洗溶液的效果：(1)用0.5%台盼蓝溶液对处理的细胞进行活体染色，(2)评估处理的细胞释放的乳酸脱氢酶的量，以及(3)对处理的犬成纤维细胞的苏木精和伊红染色的单层进行细胞病理学评估。将细胞暴露于灌洗处理以下时间间隔：0.5分钟、1分钟、2.5分钟、5分钟和10分钟。使用 PBS 作为对照。
结果
无菌自来水在所有时间间隔均显着损害犬成纤维细胞 (P = .05)。这是由于所用自来水的 pH 值呈碱性、渗透压低以及存在大量细胞毒性微量元素。暴露于生理盐水 10 分钟后，成纤维细胞出现细胞毒性作用；这可能是因为生理盐水的pH值呈酸性且缺乏缓冲系统。乳酸林格氏液不会引起任何明显的成纤维细胞损伤。
结论
PBS 和林格氏乳酸盐不会引起任何明显的成纤维细胞损伤，而生理盐水和无菌自来水在体外会引起轻微和严重的细胞毒性作用。
临床相关性
需要进一步的临床研究来确定与生理盐水相比，乳酸林格氏液是否是伤口灌洗液的首选。无菌自来水可能会导致严重的成纤维细胞损伤。
OBJECTIVE
The purpose of this study was to determine the effects of phosphate-buffered saline (PBS), sterile tap water, normal saline, and Ringer's lactate on wound healing in an in vitro model.
STUDY DESIGN
The effects of PBS, sterile tap water, normal saline, and Ringer's lactate on a primary line of canine embryonic fibroblasts were determined.
ANIMALS OR SAMPLE POPULATION
A primary line of canine embryonic fibroblasts.
METHODS
The effects of the various lavage solutions were determined by (1) vital staining of the treated cells with a 0.5% trypan blue solution, (2) evaluation of the amount of lactate dehydrogenase released by the treated cells, and (3) cytopathologic evaluation of hematoxylin and eosin-stained monolayers of treated canine fibroblasts. The cells were exposed to the lavage treatments for the following time intervals: 0.5 minute, 1 minute, 2.5 minutes, 5 minutes, and 10 minutes. PBS was used as the control.
RESULTS
Sterile tap water significantly damaged canine fibroblasts at all time intervals (P = .05). This was attributed to the alkaline pH, hypotonicity, and presence of numerous cytotoxic trace elements in the tap water used. Cytotoxic effects were noted in fibroblasts after 10 minutes' exposure to normal saline; this may be because of the acidic pH of normal saline and lack of a buffering system. Ringer's lactate did not induce any significant fibroblast injury.
CONCLUSIONS
PBS and Ringer's lactate do not induce any significant fibroblast injury, whereas normal saline and sterile tap water cause mild and severe cytotoxic effects in vitro.
CLINICAL RELEVANCE
Further clinical investigation is indicated to establish whether Ringer's lactate is the wound lavage solution of choice compared with normal saline. Sterile tap water may cause considerable fibroblast injury.