Ionic-Liquid-Based Infrared-Assisted Extraction (IL-IRAE) Coupled with HPLC-MS: a Green and Convenient Tool for Determination of TCMs
Ionic-Liquid-Based Infrared-Assisted Extraction (IL-IRAE) Coupled with HPLC-MS: a Green and Convenient Tool for Determination of TCMs
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离子液体红外辅助萃取 (IL-IRAE) 联用 HPLC-MS：绿色便捷的中药测定工具
DOI：
10.1007/s10337-016-3230-x
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发表时间：
2017-02-01
期刊：
影响因子：
1.7
通讯作者：
Yu, Yingjia
中科院分区：
文献类型：
--
作者：
Xu, Chen;Ling, Li;Yu, Yingjia
红外辐射是电磁波的一种形式，由于其加热效应而具有潜在的价值，但近年来已成为一个严重关注的问题。建立了离子液体红外辅助萃取（IL-IRAE）-高效液相色谱（HPLC）-质谱（MS）联用技术同时测定中药石蒜中3种生物碱的绿色方法。通过正交设计和方差分析，对IL的种类和浓度、提取溶剂、液固比、提取时间、辐照功率等提取工艺参数进行优化。在TSK凝胶C-18柱（150 mm x 4.6 mm id，5 μ m粒径）上以阳离子模式进行色谱-质谱分析，分离时间小于5分钟。方法的评价结果表明，分析回收率为100.66%至103.90%。在本研究考察的操作参数下，与传统的热回流提取（HRE）、超声辅助提取（UAE）和微波辅助提取（MAE）方法相比，IL-IRAE的提取效率提高了25%，表明所开发的IL-IRAE方法是环境友好、简单、低成本和高效的。为中药和天然植物中活性成分的快速测定提供了很大的希望。
Infrared radiation, one form of electromagnetic wave, has potential value due to its heating effect, but has become a matter of grave concern in recent years. We describe herein ionic-liquid-based infrared-assisted extraction (IL-IRAE) coupled with high-performance liquid chromatography (HPLC)-mass spectrometry (MS) as a green and convenient method for simultaneous determination of three alkaloids in Lycoris radiata, a traditional Chinese medicine (TCM). Extraction parameters including the type and concentration of IL, extraction solvent, liquid/solid ratio, extraction time, and irradiation power were optimized via orthogonal design and analysis of variance (ANOVA). Chromatography-mass spectrometry was performed in positive ion mode on a TSK gel C-18 column (150 mm x 4.6 mm id, 5-A mu m particle size) with less than 5-min separation. Evaluation of the method showed good results with analytical recovery ranging from 100.66 to 103.90 %. The extraction efficiency of IL-IRAE was proved to be increased by 25 % compared with conventional heat reflux extraction (HRE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) methods under the operational parameters investigated in the study, indicating that the developed IL-IRAE method is environmentally friendly, simple, low cost, and efficient, offering great promise for quick determination of active compounds in TCMs and natural plants.