Engineering the stem cell microenvironment
Engineering the stem cell microenvironment
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DOI：
10.1021/bp060350a
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Palecek, Sean P.
中科院分区：
文献类型：
--
作者：
Metallo, Christian M.;Mohr, Jeffrey C.;Palecek, Sean P.
体内的多能干细胞促进组织再生、生长和伤口愈合。它们所处的微环境提供了指导这些祖细胞增殖、分化或保持休眠的信号;这些因素包括可溶性分子、细胞外基质、邻近细胞和物理刺激。胚胎干细胞和成体祖细胞培养的最新进展需要增加对这些现象的理解。在这里，我们总结了干细胞和它们的局部环境之间的相互作用，借鉴体内观察和组织培养研究。此外，我们描述了表征各种环境因素影响的新方法，并回顾了使科学家和工程师能够更有效地指导干细胞命运的新技术。
Multipotent stem cells in the body facilitate tissue regeneration, growth, and wound healing throughout life. The microenvironment in which they reside provides signals that direct these progenitors to proliferate, differentiate, or remain dormant; these factors include soluble molecules, the extracellular matrix, neighboring cells, and physical stimuli. Recent advances in the culture of embryonic stem cells and adult progenitors necessitate an increased understanding of these phenomena. Here, we summarize the interactions between stem cells and their local environment, drawing on in vivo observations and tissue culture studies. In addition, we describe novel methods of characterizing the effects of various environmental factors and review new techniques that enable scientists and engineers to more effectively direct stem cell fate.