Role of GPR120 in cell motile activity induced by 12-O-tetradecanoylphorbol-13-acetate in liver epithelial WB-F344 cells
Role of GPR120 in cell motile activity induced by 12-O-tetradecanoylphorbol-13-acetate in liver epithelial WB-F344 cells
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DOI：
10.1007/s11010-014-2270-5
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发表时间：
2015-02-01
影响因子：
4.3
通讯作者：
Tsujiuchi, Toshifumi
中科院分区：
文献类型：
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作者：
Ishii, Shuhei;Hirane, Miku;Tsujiuchi, Toshifumi
G蛋白偶联受体120(GPR120)是不饱和长链游离脂肪酸的G蛋白偶联受体，介导胰岛素信号转导和抗炎作用。最近有报道称，GPR120促进了癌细胞的运动活性和血管生成。在本研究中，我们研究了GPR120在12-O-十四酰佛波醇-13-乙酸酯(TPA)诱导的大鼠肝上皮细胞WB-F344细胞运动活性中的作用。用5 nM的TPA处理细胞72h，实时定量RT-PCR检测Gpr120基因的表达水平。TPA处理的细胞与未处理的细胞相比，Gpr120的表达增加。在细胞运动试验中，TPA处理组的细胞运动活性明显高于未处理组。为了确定GPR120是否参与TPA介导的细胞运动活动，我们从WB-F344细胞中获得了GPR120基因敲除细胞。GPR120基因敲除可显著抑制TPA诱导的细胞运动。提示GPR120在TPA诱导WB-F344细胞运动中起重要作用。
G-protein-coupled receptor 120 (GPR120) is identified as a G-protein-coupled receptor for unsaturated long-chain free fatty acids that mediates insulin signaling and anti-inflammatory effects. Recently, it has been reported that GPR120 promotes the cell motile activity and angiogenesis in cancer cells. In this study, we assessed the role of GPR120 in the cell motile activity induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in rat liver epithelial WB-F344 cells. Cells were treated with TPA at a concentration of 5 nM for 72 h. The expression level of the Gpr120 gene was measured by quantitative real-time RT-PCR analysis. Cells treated with TPA showed the elevated Gpr120 expression, in comparison with untreated cells. In cell motility assays, the cell motile activity of cells treated with TPA was significantly higher than that of untreated cells. To confirm whether GPR120 is involved in the cell motile activity mediated by TPA, we generated GPR120 knockdown cells from WB-F344 cells. The cell motile activity induced by TPA was significantly suppressed by GPR120 knockdown. These results suggest that GPR120 plays an important role in the cell motile activity induced by TPA in WB-F344 cells.