Neuropharmacological consequences of choline administration
Neuropharmacological consequences of choline administration
复制标题
胆碱给药的神经药理学后果
DOI：
10.1016/0006-8993(80)90605-8
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
D. Schmidt
中科院分区：
文献类型：
--
作者：
L. Wecker;D. Schmidt
Recent studies have shown that acute choline administration prevents atropineinduced acetylcholine (ACh) depletion in rat cerebral cortex and hippocampus, but the mechanisms involved have not been elucidated la. The effects of atropine on brain levels of ACh are presumably due to increased neurotransmitter release relative to synthesis1, 2, 4, 10 and it has been suggested that the prophylactic actions of exogenous choline may involve alterations in ACh synthesis 1~. In light of evidence indicating that the utilization of exogenous choline and the regulatory mechanisms controlling the synthesis of ACh in the striatum differ from other brain regions 9, x2, we investigated and compared the effects of choline pretreatment on dose-dependent atropine-induced ACh depletion in rat striatum and hippocampus. Male Sprague-Dawley rats (180-230 g) were maintained on a 12 h light/dark cycle with standard rat chow (Wayne Lab Blox MRH 22/5, Allied Mills, Chicago, I11.) and water available ad libitum. All drugs were dissolved in saline and administered intraperitoneally (0.1 ml/100 g body weight) between 09.00 and 11.00. Animals were killed by microwave irradiation (2.4 sec) focused on the head in a modified Litton model 70-50 oven (2750 MHz, 1250 W). Under these conditions, the brain temperature reached 90 C and all enzymes were inactivated 6. Following sacrifice, brains were removed, chilled in ice-cold pentane and dissected into discrete brain areas using a brain slicing apparatus s. Slices from the caudate-putamen and hippocampus were isolated, homogenized (Teflon-glass) in acetonitrile containing propionylcholine iodide as an internal standard and prepared for the simultaneous determination of ACh and choline by pyrolysis-gas chromatography7, 11.Choline time course effects were determined in brain regions from rats injected with 60-120 mg/kg (free base) choline iodide and sacrificed 15-90 min following injection. Results were calculated as nmol ACh or choline/g brain tissue and expressed as group mean values~-SEM The effects of choline on atropine-induced ACh depletion were studied in animals injected with saline or choline iodide (60 mg/kg, free
