Fluorescent Gold Nanoprobe Sensitive to Intracellular Reactive Oxygen Species
Fluorescent Gold Nanoprobe Sensitive to Intracellular Reactive Oxygen Species
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DOI：
10.1002/adfm.200801838
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发表时间：
2009-06-23
影响因子：
19
通讯作者：
Park, Tae Gwan
中科院分区：
文献类型：
--
作者：
Lee, Hyukjin;Lee, Kyuri;Park, Tae Gwan
制备固定有荧光素-透明质酸（HA）缀合物的金纳米探针，并用于通过纳米颗粒表面能量转移监测活细胞中的细胞内活性氧（ROS）产生。一种生物启发的粘附分子，多巴胺，被用来鲁棒性地将end-HA连接到金纳米颗粒（AuNPs）的表面上，以确保细胞内对谷胱甘肽的稳定性。ROS诱导固定在AuNP表面上的HA链的切割和片段化，允许通过发射强荧光恢复信号来快速和特异性地检测细胞内ROS。特别地，荧光猝灭的金纳米探针在暴露于某些氧物质如过氧化阴离子（O-2（-））和羟基自由基（OH）时表现出选择性和剂量依赖性的荧光恢复信号。荧光金纳米探针可用于实时细胞内ROS检测和抗氧化剂筛选分析，并作为一类新的ROS成像探针在各种生物医学应用中具有令人兴奋的潜力。
Gold nanoprobes immobilized with fluorescein-hyaluronic acid (HA) conjugates are fabricated and utilized for monitoring intracellular reactive oxygen species (ROS) generation in live cells via nanoparticle surface energy transfer. A bio-inspired adhesive molecule, dopamine, is used to robustly end-immobilize HA onto the surface of gold nanoparticles (AuNPs) for securing intracellular stability against glutathione. ROS induces cleavage and fragmentation of the HA chains immobilized on the surface of the AuNPs allows rapid and specific detection of intracellular ROS by emitting strong fluorescence-recovery signals. in particular, fluorescence-quenched gold nanoprobes exhibit selective and dose-dependent fluorescence-recovery signals upon exposure to certain oxygen species such as surperoxide anion (O-2(-)) and hydroxyl radical (OH). The fluorescent gold nanoprobe is usefully exploited for real-time intracellular ROS detection and antioxidants screening assay, and has exciting potential for various biomedical applications as a new class of ROS imaging probes.