Epithelium of the human vocal fold as a vibrating tissue
Epithelium of the human vocal fold as a vibrating tissue
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人类声带上皮作为振动组织
DOI：
10.1016/j.anl.2020.11.011
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发表时间：
2021
影响因子：
1.7
通讯作者：
Umeno H
中科院分区：
文献类型：
--
作者：
Sato K;Chitose S;Sato K;Sato F;Ono T;Umeno H
目的成人声带粘膜，尤其是固有层(Reinke‘s间隙)的浅层，作为一种重要的振动结构已引起人们的广泛关注。然而，成人声带黏膜的另一个组织成分--复层扁平上皮的精细结构仍然是个谜。方法对3例正常成人声带和会厌及3例新生声带进行观察。结果成人声带上皮最明显的特征是相邻上皮细胞间隙较大(984±1186 nm)，而成人会厌会厌复层鳞状上皮和新生声带上皮细胞间隙较大。成人声带相邻上皮细胞间隙较大，但相邻上皮细胞交界处的桥粒形成了牢固的细胞间黏附。张力细丝由固定在桥粒上的中间细丝束组成。桥粒形成一个连续的细胞骨架网络，遍及成人声带的上皮细胞和上皮组织。此外，成人声带上皮细胞之间存在大量的E-钙粘附素(粘附性糖蛋白)，尤其是成人声带复层鳞状上皮的下半部。结论从功能形态上看，成人声带复层鳞状上皮似乎形成了一种抗张强度高、柔韧性好、适于振动的结构框架。
ObjectivesThe human adult vocal fold mucosa, especially, superficial layer of the lamina propria (Reinke's space) has attracted notice as an important vibrating structure. However, fine structures of the stratified squamous epithelium of the human adult vocal fold, which is another histological component of the mucosa, remain enigmatic.MethodsThree normal human adult vocal folds and epiglottises and three newborn vocal folds were investigated. Observations using transmission electron microscopy and light microscopy including immunohistochemistry were performed.ResultsThe most obvious feature of the epithelium of the human adult vocal folds was that the intercellular spaces between adjacent epithelial cells were large (984 ± 186 nm) compared with the stratified squamous epithelium of the human adult epiglottis and the human newborn vocal fold.Even though intercellular spaces between adjacent epithelial cells of the human adult vocal fold were large, desmosomes at the junctions of two adjacent epithelial cells made firm intercellular adhesion. Tonofilaments composed of the bundles of intermediate filaments anchored to the desmosomes. Desmosomes formed a continuous cytoskeletal network throughout the epithelial cells and epithelium of the human adult vocal fold. In addition, a great deal of E-cadherin (adhesive glycoprotein) was present between epithelial cells especially the lower half of the stratified squamous epithelium of the human adult vocal fold.ConclusionsFrom the functional morphological point of view, stratified squamous epithelium of the human adult vocal fold seems to form a structural framework of tensile strength with pliability suitable structure for vibration.