YY1 repressing peroxisome proliferator-activated receptor delta promoter
YY1 repressing peroxisome proliferator-activated receptor delta promoter
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DOI：
10.1007/s11010-007-9632-1
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Fan, Wei
中科院分区：
文献类型：
--
作者：
He, Cheng-Qiang;Ding, Nai-Zheng;Fan, Wei
过氧化物酶体增殖物激活受体δ（Peroxisome proliferator-activated receptors delta，PPARdelta）是一种核激素受体，属于类固醇受体超家族，是治疗高脂血症和2型糖尿病药物的分子靶点。Yin Yang 1（YY 1）是一种转录因子，可以抑制或激活与其相互作用的基因的转录。在这份报告中，我们表明，YY 1特异性相互作用的PPAR δ启动子。在Hela和NIH 3 T3细胞中，YY 1的过表达抑制了PPAR δ启动子的活性，而当用siRNA YY 1敲低YY 1时，PPAR δ启动子的活性增强。我们还表明，核提取物中的YY 1能够直接结合的PPAR δ启动子。这些结果表明，YY 1可能是一个负调控因子的PPAR δ基因表达，通过其直接相互作用与PPAR δ启动子。
Peroxisome proliferator-activated receptors delta (PPAR delta) is a nuclear hormone receptor belonging to the steroid receptor superfamily and is molecular targets for drugs to treat hypertriglyceridemia and type 2 diabetes. Yin Yang 1 (YY1) is a transcription factor that can repress or activate transcription of the genes with which it interacts. In this report, we show that YY1 specifically interacts with the PPAR delta promoter. Overexpression of YY1 in Hela and NIH 3T3 cells repressed the activity of the PPAR delta promoter, while the PPAR delta promoter activity was enhanced when YY1 was knocked down by siRNA YY1. We also show that YY1 in nuclear extracts was able to bind the PPAR delta promoter directly. These results suggest that YY1 might be a negative regulator of PPAR delta gene expression through its direct interaction with the PPAR delta promoter.