Transactivation activity of thyroid hormone receptors in fish (Conger myriaster) in response to thyroid hormones
Transactivation activity of thyroid hormone receptors in fish (Conger myriaster) in response to thyroid hormones
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DOI：
10.1016/j.cbpb.2006.05.003
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发表时间：
2006-08-01
影响因子：
2.2
通讯作者：
Ohta, Hiromi
中科院分区：
文献类型：
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作者：
Kawakami, Yutaka;Shin, Dong-Hwan;Ohta, Hiromi
先前分离的编码海鳗（Conger myriaster）甲状腺激素（TH）受体（TR）的cDNA。在本研究中，我们研究了用TH（3，3 '，5-triiodo-L-thyronine [T-3]、L-甲状腺素[T-4]和3，3'，5 '-triiodo-L-thyronine [rT（3）]）或其他核受体的配体和激活剂处理的海鳗TRs的反式激活活性。瞬时转染日本鳗鲡肝细胞系Hepa-E1后，海鳗TRs（cTRs）显示TH依赖性激活TH应答启动子的转录。然而，没有任何的四个cTR的反式激活活性与其他核受体的配体或激活剂观察。虽然T-3对cTRs的激活作用强于其他TH（T-3 > T-4 > rT（3）），但其反式激活敏感性与cTRs对TH的活性不同。因此，我们得出结论，cTRs可以与TH在鱼类变态中协同作用。(c)2006年爱思唯尔公司All rights reserved.
previously isolated cDNAs encoding conger eel (Conger myriaster) thyroid hormone (TH) receptors (TRs). In the present study, we investigated the transactivation activities of conger eel TRs treated with THs (3,3',5-triiodo-L-thyronine [T-3], L-thyroxine [T-4], and 3,3',5'-triiodo-L-thyronine [rT(3)]), or ligands and activators of other nuclear receptors. Following transient transfection into the Japanese eel (Anguilla japonica) hepatocyte cell line Hepa-E1, the conger eel TRs (cTRs) showed TH-dependent activation of transcription from a TH-responsive promoter. However, no transactivation activity of any of the four cTRs was observed with ligands or activators of other nuclear receptors. Although T-3 activation for cTRs is stronger than other THs (T-3 > T-4 > rT(3)), the transactivation sensitivity was different from the activity of cTRs with THs, respectively. Therefore, we conclude that cTRs can act in concert with THs in fish metamorphosis. (c) 2006 Elsevier Inc. All rights reserved.