An anaerobic dynamic membrane bioreactor (AnDMBR) for landfill leachate treatment: Performance and microbial community identification

An anaerobic dynamic membrane bioreactor (AnDMBR) for landfill leachate treatment: Performance and microbial community identification
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DOI:
10.1016/j.biortech.2014.03.014
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发表时间:
2014-06-01
影响因子:
11.4
通讯作者:
Wu, Zhichao
Wu, Zhichao
中科院分区:
工程技术1区
文献类型:
--
作者:
Xie, Zhenfang;Wang, Zhiwei;Wu, Zhichao

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采用厌氧动态膜生物反应器处理垃圾渗滤液,进行了为期142天的中试试验。在稳定运行条件下,当进水总氨氮浓度大于3000 mg/L,COD浓度大于13,000 mg/L时,反应器对COD的平均去除率可达62.2%。在有机负荷为4.87kgCOD/(m3·d)时,沼气中甲烷含量为70- 90%,平均产甲烷量为0.34L/gCOD。焦磷酸测序分析表明,在运行过程中,古菌群落相对稳定,而细菌群落发生了明显变化。在细菌群落中,嗜碱菌、Petrimonas、Fastidiosipila和vadinBC 27是最丰富的发酵细菌。此外,TM 6门逐渐成为最优势的细菌群落,并达到最高的相对丰度为32.9%,随着操作的推移。在古菌群落中,甲烷八叠球菌属被确定为优势产甲烷菌。(C)2014爱思唯尔有限公司版权所有。
In this study, a pilot-scale anaerobic dynamic membrane bioreactor was operated for 142 days for treating landfill leachate. Under stable operation, average COD removal efficiency of 62.2% was achieved when the reactor was fed with the raw leachate containing total ammonium concentration above 3000 mg/L and COD above 13,000 mg/L. The methane content in the biogas was in the range of 70-90%, and the average methane yield was 0.34 L/g CODremoved at the organic loading rate of 4.87 kg COD/(m(3) d). Pyrosequencing analyses indicated that during the operation the archaeal community was relatively stable while obvious changes took place in the bacterial community. Alkaliphilus, Petrimonas, Fastidiosipila and vadinBC27 were the abundant fermentation bacteria in bacterial communities. Moreover, phylum TM6 gradually became the most dominant bacterial community and reached the highest relative abundance of 32.9% as the operation elapsed. In archaeal communities, genus Methanosarcina was identified as the dominant methanogen. (C) 2014 Elsevier Ltd. All rights reserved.