Electron microscope studies of experimental Entamoeba histolytica infection in the guinea pig. I. Penetration of the intestinal epithelium by trophozoites.
Electron microscope studies of experimental Entamoeba histolytica infection in the guinea pig. I. Penetration of the intestinal epithelium by trophozoites.
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豚鼠实验性溶组织内阿米巴感染的电子显微镜研究。
DOI：
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发表时间：
1975
影响因子：
3.3
通讯作者：
B. P. Phillips
中科院分区：
文献类型：
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作者：
A. Takeuchi;B. P. Phillips
无菌豚鼠接种肠内溶组织内阿米巴和肠道菌群来源于急性阿米巴结肠炎患者。接种后间隔7 ~ 12天处死动物。光镜和电镜观察阿米巴对盲肠上皮的侵彻方式。下面的序列是根据许多单独的观察结果重建的。当变形虫离丛缘较近时，微绒毛变短，不规则，有时消失。变形虫与微绒毛之间有致密物质。当变形虫非常靠近上皮时，上皮细胞质的顶端部分投射到与生物体接触的管腔中，从而与相邻细胞分离。这在上皮细胞之间产生了间隙，变形虫通过这个间隙侵入上皮细胞间隙。最初，阿米巴穿透腺间上皮。后来，它同样穿透腺体和腺体间上皮屏障。上皮细胞的细胞质成分有明显改变。多形核白细胞迁移到上皮内填充这些间隙；这些通常表现为各种退化过程。变形虫利用它们的伪足，通过细胞间隙进一步移动并到达固有层。
Germ-free guinea pigs were inoculated intracecally with Entamoeba histolytica and the enteric flora derived from a human patient with acute amebic colitis. Aanimals were killed at post-inoculation intervals of 7 to 12 days. The mode of penetration of cecal epithelium by the ameba was examined by light and electron microscopy. The following sequence was reconstructed from numerous individual observations. When the amebae were in moderately close proximity to the brush, border, the microvilli became shortened, irregular, and sometimes disappeared. Dense material was observed between the amebae and microvilli. When the ameba was very close to the epithelium the apical portion of epithelial cytoplasm projected into the lumen contacting the organism, thus becoming detached from adjoining cells. This produced spaces between epithelial cells through which amebae invaded interepithelial spaces. Initially the ameba penetrated the interglandular epithelium. Later, it penetrated equally the glandular and interglandular epithelium barrier. There were marked alterations of cytoplasmic components of epithelial cells. Polymorphonuclear leukocytes migrated into the epithelium filling these spaces; these often showed a variety of degenerative processes. Amebae, utilizing their pseudopodia, moved further through the intercellular spaces and reached the lamina propria.