Classification of NPY-expressing neocortical interneurons.
Classification of NPY-expressing neocortical interneurons.
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DOI：
10.1523/jneurosci.0058-09.2009
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发表时间：
2009-03-18
期刊：
影响因子：
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通讯作者：
Cauli B
中科院分区：
文献类型：
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作者：
Karagiannis A;Gallopin T;Dávid C;Battaglia D;Geoffroy H;Rossier J;Hillman EM;Staiger JF;Cauli B
神经肽Y（NPY）是涉及大量物理和病理过程的新皮层的大量神经肽，这是由于较大的电生理学，分子和表达NPY神经元的形态多样性，我们的精确身份仍然不清楚。特征是在大鼠桶皮层的急性切片中，通过全细胞贴剂夹夹200层I – IV的皮质神经元记录，生物细胞蛋白标记和单细胞逆转录酶PCR旨在探测良好的皮质神经元分子标记，以对可靠的皮质NPY神经元进行分类，我们使用并比较了基于层层次和电力物理学的位置和电子生理生理学的位置和电力物理学的算法，分子特性。披露了表达NPY的三种主要表达神经元。升节的轴突在I层上均匀表达生长抑制素，是快速刺激性和白细胞蛋白阳性篮细胞中最令人兴奋的类型。表达与明显的尖峰潜伏期相关。
Neuropeptide Y (NPY) is an abundant neuropeptide of the neocortex involved in numerous physiological and pathological processes. Because of the large electrophysiological, molecular, and morphological diversity of NPY-expressing neurons their precise identity remains unclear. To define distinct populations of NPY neurons we characterized, in acute slices of rat barrel cortex, 200 cortical neurons of layers I–IV by means of whole-cell patch-clamp recordings, biocytin labeling, and single-cell reverse transcriptase-PCR designed to probe for the expression of well established molecular markers for cortical neurons. To classify reliably cortical NPY neurons, we used and compared different unsupervised clustering algorithms based on laminar location and electrophysiological and molecular properties. These classification schemes confirmed that NPY neurons are nearly exclusively GABAergic and consistently disclosed three main types of NPY-expressing interneurons. (1) Neurogliaform-like neurons exhibiting a dense axonal arbor, were the most frequent and superficial, and substantially expressed the neuronal isoform of nitric oxide synthase. (2) Martinotti-like cells characterized by an ascending axon ramifying in layer I coexpressed somatostatin and were the most excitable type. (3) Among fast-spiking and parvalbumin-positive basket cells, NPY expression was correlated with pronounced spike latency. By clarifying the diversity of cortical NPY neurons, this study establishes a basis for future investigations aiming at elucidating their physiological roles.