Microsatellite markers for diverse Salix species
Microsatellite markers for diverse Salix species
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DOI：
10.1046/j.1471-8286.2003.00332.x
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发表时间：
2003-03-01
期刊：
影响因子：
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通讯作者：
Karp, A
中科院分区：
文献类型：
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作者：
Barker, JHA;Pahlich, A;Karp, A
从丰富的黑柳文库中分离出 46 个微卫星，并在国家柳树保藏中心（英国 IACR-朗阿什顿研究站）的 20 个个体（9 个物种/杂交种）上进行了测试。二十九个是单态的，给出多位点或不可评分的模式，或者是重复的。其余 17 个微卫星提供 2-22 个等位基因/基因座。三个微卫星在另外 31 个柳属物种中成功交叉扩增。另外 6 个在由 31 个物种的 6-25 个个体组成的小组中进行了测试。在所有情况下交叉扩增都是成功的。这些结果表明，这里分离的微卫星应该对多种柳属物种的种群研究有用。
Forty-six microsatellites were isolated from an enriched library of Salix butjatica and tested on 20 individuals (of nine species/hybrids) from the National Willows Collection (IACR-Long Ashton Research Station, UK). Twenty-nine were monomorphic, gave multilocus or unscorable patterns, or were duplicates. The remaining 17 microsatellites gave 2-22 alleles/ locus. Three microsatellites successfully cross-amplified in 31 additional Salix species. A further six were tested on panels comprising 6-25 individuals from the 31 species. Cross-amplification was successful in all cases. These results suggest that the microsatellites isolated here should prove useful for population studies in a wide range of Salix species.