Identification of Three Circular RNA Cargoes in Serum Exosomes as Diagnostic Biomarkers of Non-Small-Cell Lung Cancer in the Chinese Population
Identification of Three Circular RNA Cargoes in Serum Exosomes as Diagnostic Biomarkers of Non-Small-Cell Lung Cancer in the Chinese Population
复制标题
血清外泌体中三种环状 RNA 货物的鉴定作为中国人群非小细胞肺癌的诊断生物标志物
DOI：
10.1016/j.jmoldx.2020.05.011
复制
发表时间：
2020-08-01
影响因子：
4.1
通讯作者：
Yang, Lei
中科院分区：
文献类型：
--
作者：
Xian, Jianfeng;Su, Wenpeng;Yang, Lei
最近在血清外切体中发现的环状RNAs(CircRNAs)为非侵袭性癌症诊断提供了一种新的和潜在的有用工具。然而，目前还没有针对这一主题的实质性研究。对3对非小细胞肺癌(NSCLC)患者和对照组进行了RNA测序分析。在训练集、验证集1和验证集2中评估了外体CircRNA的诊断价值。总共鉴定了46个异常表达的CircRNA。其中，CIRC_0047921、CIRC_0056285和CIRC_0007761对非小细胞肺癌具有显著的诊断有效性。CIRC_0047921可区分非小细胞肺癌和慢性阻塞性肺疾病对照组(AUC，0.890；95%CI，0.831-0.948)，而CIRC_0056285和CIRC_0007761组合可区分非小细胞肺癌和慢性阻塞性肺疾病(AUC，0.890；95%CI，0.831-0.948)。95%可信区间，0.739-0.902)。对于早期NSCLC诊断，这些CircRNAs在区分早期NSCLC病例与健康对照组、慢性阻塞性肺疾病对照组或结核病对照组方面也观察到了类似的结果。CIRC_0056285的表达与临床分期和淋巴结转移有关。CIRC_0047921、CIRC_0056285和CIRC_0007761是中国人群诊断非小细胞肺癌很有前景的生物标志物。
The recent discovery of circular RNAs (circRNAs) in serum exosomes suggests a novel and potentially useful tool for noninvasive cancer diagnosis. However, there are currently no substantial studies addressing this topic. RNA-sequencing analysis was performed between three pairs of non-small-cell lung cancer (NSCLC) patients and controls. The diagnostic values of exosomal circRNAs were assessed in a training set, validation set 1, and validation set 2. A total of 46 abnormally expressed circRNAs were identified. Among these, circ_0047921, circ_0056285, and circ_0007761 were found to display significant diagnostic validity for NSCLC. In distinguishing NSCLC cases from healthy controls, the panel of aforementioned circRNAs exhibited area under the curve (AUC) values of 0.926 (95% CI, 0.895-0.956) in the training set and 0.919 (95% CI, 0.877-0.962) in validation set 1. circ_0047921 could distinguish NSCLC cases from chronic obstructive pulmonary disease controls (AUC, 0.890; 95% CI, 0.831-0.948), whereas the circ_0056285 and circ_0007761 combination could distinguish NSCLC cases from tuberculosis controls (AUC, 0.820; 95% CI, 0.739-0.902). For an early NSCLC diagnosis, similar results were observed for these circRNAs in distinguishing early-stage NSCLC cases from healthy controls, chronic obstructive pulmonary disease controls, or tuberculosis controls. circ_0056285 expression was correlated with clinical stages and lymph node metastasis. The exosomal circRNAs, circ_0047921, circ_0056285, and circ_0007761, are promising biomarkers for NSCLC diagnosis in the Chinese population.