Expression of peripherin in the pig spiral ganglion - aspects of nerve injury and regeneration
Expression of peripherin in the pig spiral ganglion - aspects of nerve injury and regeneration
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DOI：
10.1080/00016480802369294
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发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Rask-Andersen, Helge
中科院分区：
文献类型：
--
作者：
Liu, Wei;Bostrom, Marja;Rask-Andersen, Helge
结论Peripherin蛋白不仅对发育中的神经元，而且对成熟耳蜗中轴突突起的维持和再生都很重要。对它的表达和功能的进一步了解有助于我们对损伤耳蜗轴突再生和神经修复的理解。Objective.研究外周蛋白在成年猪螺旋神经节和培养的豚鼠螺旋神经节神经元中的存在。材料与方法。用抗外周蛋白抗体对成年猪螺旋神经节和豚鼠螺旋神经节细胞（SGC）培养物的切片进行免疫组织化学。结果在猪听觉神经元中，I型和II型SGCs均表现出外周蛋白的表达。这些细胞并不优先位于神经节内螺旋束（IGSB）附近。IGSB由显示强烈外周蛋白的细口径纤维和TUJ-1阳性的较粗纤维组成。IGSB内只有少数纤维同时表达外周蛋白和TUJ-1。培养的豚鼠神经元显示出丰富的表达外周蛋白，包括核。这种蛋白质表达的区域，如核周体和轴突，但也有一个节段性表达的外周蛋白在某些地区。Peripherin在轴突分支区域和活跃生长锥和lammelipodia的中心表达更多。
Conclusion. Peripherin protein may be important not only for developing neurons but also for the maintenance and regeneration of axonal processes in the mature cochlea. More knowledge about its expression and function could improve our understanding with reference to axonal regrowth and nerve restoration in the damaged cochlea. Objective. To investigate the existence of peripherin protein in adult pig spiral ganglion and cultured spiral ganglion neurons of the guinea pig. Materials and methods. Immunohistochemistry with anti-peripherin antibodies was performed on sections of adult pig spiral ganglion and guinea pig spiral ganglion cell (SGC) culture. Results. In pig auditory neurons, both type I and type II SGCs showed expression of the protein peripherin. These cells were not preferentially located near the intraganglionic spiral bundle (IGSB). The IGSB consisted of thin calibre fibres showing intense peripherin and thicker fibres that were TUJ-1 positive. Only a few fibres within the IGSB co-expressed both peripherin and TUJ-1. Cultured guinea pig neurons displayed a rich expression of peripherin, including the nuclei. This protein was expressed in regions such as the perikaryon and axons but there was also a segmental expression of peripherin in some regions. Peripherin was more expressed in areas of axon branching and in the centre of the active growth cones and lammelipodia.