Changing medium and passaging cell lines
Changing medium and passaging cell lines
复制标题
DOI：
10.1038/nprot.2007.319
复制
发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Stacey, Glyn N.
中科院分区：
文献类型：
--
作者：
Masters, John R.;Stacey, Glyn N.
细胞系广泛应用于生物医学研究。本规程描述了常规改变培养基和传代细胞的方法。培养基的变化通过提供新鲜的营养物质来保持细胞的健康，而细胞传代或分裂则需要维持细胞的指数增长。尽管使用的方法很简单，但每个细胞系都有自己的特点。无论是使用一种还是几种细胞系，都无法替代对它们需求的了解，包括在不同物理和营养条件下的表型范围和生长模式。给予必要的照顾和关注，大多数细胞系很容易维持和生长。
Cell lines are widely used in biomedical research. This protocol describes the methods used routinely to change the medium and passage the cells. Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth. Despite the simplicity of the methods used, each cell line has idiosyncracies. Whether working with one or several cell lines, there is no substitute for knowledge of their needs, including the range of phenotypes and growth patterns under different physical and nutrient conditions. Given the necessary care and attention, most cell lines are easy to maintain and grow.