MIGRATORY ACTIVITY OF BLOOD POLYMORPHONUCLEAR LEUKOCYTES DURING JUVENILE RHEUMATOID-ARTHRITIS, DEMONSTRATED WITH A NEW WHOLE-BLOOD MEMBRANE-FILTER ASSAY
MIGRATORY ACTIVITY OF BLOOD POLYMORPHONUCLEAR LEUKOCYTES DURING JUVENILE RHEUMATOID-ARTHRITIS, DEMONSTRATED WITH A NEW WHOLE-BLOOD MEMBRANE-FILTER ASSAY
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DOI：
10.1007/bf01534440
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发表时间：
1994-08-01
期刊：
影响因子：
5.1
通讯作者：
KENZIAN, H
中科院分区：
文献类型：
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作者：
EGGER, G;KLEMT, C;KENZIAN, H
在由膜滤器（3 μ m孔，140 μ m厚）组成的迁移室中测量全血中的多形性白细胞（PMN）迁移，该膜滤器具有与塑料容器连接的整合的化学引诱物储库（固体形式的FMLP）。对照室缺少FMLP（空白）。一个测试单元需要300 μ l血液。通过显微镜确定进入过滤器的PMN移民的数量和分布。共26个测量的PMN迁移在5个幼年类风湿关节炎（JRA）患者不同的疾病活动进行了比较与健康对照组（N = 32）的反应。相关性计算与常规实验室参数（WBC，PLT，BSR，CRP，Hgb，血清Fe）和疾病活动。与健康对照组相比，JRA患者的PMN通常显示出显著增加的渗透深度进入过滤器，无论是否存在化学引诱物或疾病活动。仅在高疾病活动期间发现与空白相比对FMLP的迁移反应增加。PMN穿透深度与CRP呈正相关，与Hgb呈正相关。迁移试验结合了快速和简单的处理与真正的PMN活化状态的良好保存。
Polymorphonuclear leukocyte (PMN) migration is measured in whole blood in a migration chamber consisting of a membrane filter (3-mu m pores, 140 mu m thick) with an integrated chemoattractant depot (FMLP in solid form) attached to a plastic container. Control chambers lack FMLP (blanks). One test unit requires 300 mu l blood. Numbers and distribution of the PMN immigrants into the filters are determined microscopically. Altogether 26 measurements of PMN migration in five juvenile rheumatoid arthritis (JRA) patients with varying disease activity were compared with the reactions of a healthy control group (N = 32). Correlations were calculated with conventional laboratory parameters (WBC, PLT, BSR, CRP, Hgb, serum Fe) and disease activity. In comparison with healthy controls, PMNs of JRA patients generally show a markedly increased penetration depth into the filters irrespective the presence of the chemoattractant or the disease activity. Increased migratory reactions to FMLP in comparison to blanks were found during high disease activity only. The PMN penetration depth correlates positively with the CRP, and reciprocally with the Hgb blood levels. The migration assay combines fast and simple processing with good preservation of the genuine PMN activation state.