Functional analysis of the seed coat-specific gene GbMYB2 from cotton
Functional analysis of the seed coat-specific gene GbMYB2 from cotton
复制标题
棉花种皮特异性基因GbMYB2的功能分析
DOI：
10.1016/j.plaphy.2013.08.004
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发表时间：
2013-12-01
影响因子：
6.5
通讯作者：
Zuo, Kaijing
中科院分区：
文献类型：
--
作者：
Huang, Yiqun;Liu, Xiang;Zuo, Kaijing
MYB转录因子是棉纤维发育所必需的。我们从海岛棉中克隆了R2 R3-MYB基因GbMYB 2。RNA原位杂交分析表明，GbMYB 2主要在棉花胚珠的外珠被和伸长纤维中表达。GbMYB 2的表达增加整个纤维的起始和在伸长阶段。GbMYB 2在陆地棉品种Xu 142中的表达水平高于Xu 142（fl）突变体。在拟南芥中过量表达GbMYB 2导致更厚的叶毛和更长的根发展，由于毛发育相关基因，如GL 2的激活。这些结果表明，GbMYB 2是一个R2 R3-MYB基因，参与纤维发育。(C)2013年Elsevier Masson SAS。All rights reserved.
MYB transcription factors are essential for cotton fiber development. We isolated the R2R3-MYB gene GbMYB2 from Gossypium barbadense. RNA in situ hybridization analysis showed that GbMYB2 is mainly expressed in the outer integuments of cotton ovules and in elongating fibers. GbMYB2 expression increased throughout fiber initiation and during the elongation stage. The expression level of GbMYB2 was higher in the Gossypium hirsutum cultivar Xu142 than in the Xu142 (fl) mutant. Overexpression of GbMYB2 in Arabidopsis caused thicker leaf trichomes and longer roots to develop due to the activation of trichome development-related genes such as GL2. These results indicate that GbMYB2 is an R2R3-MYB gene that is involved in fiber development. (C) 2013 Elsevier Masson SAS. All rights reserved.