Concentrations of faecal immunoreactive progestagen metabolites during the oestrous cycle and pregnancy in the black rhinoceros (Diceros bicornis michaeli).
Concentrations of faecal immunoreactive progestagen metabolites during the oestrous cycle and pregnancy in the black rhinoceros (Diceros bicornis michaeli).
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黑犀牛（Diceros bicornis michaeli）发情周期和妊娠期间粪便免疫反应性孕激素代谢物的浓度。
DOI：
10.1530/jrf.0.0980285
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Germany
中科院分区：
文献类型：
--
作者：
F. Schwarzenberger;R. Francke;R. Glo;Zoologischer Garlen;Hardenbergplatz Berlin;D. Berlin;Germany
粪便样本中生殖激素的分析为非侵入性监测自由放养或难驯化物种的生殖状况提供了可能。在本研究中，在发情周期和妊娠期间，大约每周一次采集三只黑犀牛(Diceros BiornisMichaeli)的粪便样本。在分娩后一周内每日采集样本。用三种不同的酶免疫分析法(EIA)分析了粪便提取物中的总免疫反应性孕激素，它们对含有20α-羟基或20-酮基的孕激素具有相当高的特异性。每次EIA都有可能区分发情周期的卵泡期和黄体期。交配对应较低浓度的粪孕激素。取自两头犀牛连续5个周期和6个周期的样本，计算出周期长度分别为24天和26.5天。所有三只动物都怀孕了，怀孕时间从440天到470天不等。受精后，黄体期孕激素浓度持续升高，在60-250天达到5-10倍。在分娩前两周，粪孕激素水平下降，产后3-4天内达到卵泡期水平。结论是，在黑犀牛的粪便中存在几种免疫活性孕激素，用EIA测量它们可以非侵入性地监测发情周期和妊娠。
The analysis of reproductive hormones in faecal samples is a possibility for non-invasive monitoring of reproductive status in free-ranging or intractable species. In the present study, faecal samples from three black rhinoceroses (Diceros bicornis michaeli) were collected at about weekly intervals during oestrous cycles and pregnancy. Daily samples were taken during the week after parturition. Total immunoreactive progestagens in faecal extracts were analysed with three different enzymeimmunoassays (EIA) that had considerable specificity for progestagens containing either a 20 alpha-hydroxyl or a 20-keto group. With each EIA it was possible to distinguish between the follicular and luteal phases of the oestrous cycle. Mating corresponded with low concentrations of faecal progestagens. Samples from five and six consecutive cycles were available from two rhinoceroses and cycle lengths of 24 and 26.5 days were calculated. All three animals became pregnant and the duration of gestation ranged from 440 to 470 days. After fertilization, the concentration of progestagens increased continuously, as in the luteal phase, reaching values 5-10 times higher between days 60 and 250. During the two weeks before parturition faecal progestagens declined and within 3-4 days post partum had reached follicular phase values. It was concluded that several immunoreactive progestagens are present in the faeces of black rhinoceroses and that their measurement with EIA enables non-invasive monitoring of the oestrous cycle and pregnancy.
快速提取粪便类固醇，用于测量自由放养的黄色狒狒（Papio cynocephalus cynocephalus）的生殖周期和早期妊娠。
DOI：
10.1530/jrf.0.0920415
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Wasser,SK;Monfort,SL;Wildt,DE
通讯作者：
Wildt,DE