WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway.
WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway.
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DOI：
10.3892/mmr.2012.860
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发表时间：
2012-07
影响因子：
3.4
通讯作者：
Ping Zhang;Renbing Jia;Lei Ying;Bo Liu;Guanxiang Qian;Xianqun Fan;S. Ge
中科院分区：
文献类型：
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作者：
Ping Zhang;Renbing Jia;Lei Ying;Bo Liu;Guanxiang Qian;Xianqun Fan;S. Ge
人类WWOX基因，被称为含有WW结构域的氧化还原酶，位于16q23.3-24.1染色体区域，跨越常见的脆性位点FRA16D。在许多癌症细胞类型中，包括乳腺癌、卵巢癌、前列腺癌和肺癌细胞，经常发现转录异常甚至表达缺失。因此有人提出，WWOX基因编码一种候选肿瘤抑制因子，可能是促凋亡蛋白。然而，这背后的机制并不完全清楚。在本研究中，我们通过在A549细胞中的瞬时表达来检测WWOX的促凋亡作用。我们观察到WWOX的异位表达导致A549细胞凋亡。我们进一步观察了转染pcDNA3.0-WWOX的A549细胞中procaspase-3和procaspase-9的活化以及线粒体中细胞色素C的释放。这些数据表明，WWOX通过线粒体途径诱导A549细胞凋亡。
The human WWOX gene, known as WW domain-containing oxidoreductase, is located on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D. Abnormal transcripts or even loss of expression are frequently found in a number of cancer cell types, including breast, ovarian, prostate and lung cancer cells. It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor, possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely clear. In the present study, we examined the pro-apoptotic action of WWOX using transient expression in A549 cells. We observed that the ectopic expression of WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9 activation and the release of cytochrome C from the mitochondria in A549 cells transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis in A549 cells via the mitochondrial pathway.