Influence of environmental and nutritional factors in the production of astaxanthin from Haematococcus pluvialis
Influence of environmental and nutritional factors in the production of astaxanthin from Haematococcus pluvialis
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DOI：
10.1016/j.biortech.2003.04.001
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发表时间：
2004-04-01
影响因子：
11.4
通讯作者：
Campocosio, AT
中科院分区：
文献类型：
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作者：
Domínguez-Bocanegra, AR;Legarreta, IG;Campocosio, AT
从绿藻中提取的虾青素由于其抗氧化特性在食品和制药工业中是理想的。绿色单细胞清水微藻雨红球菌产虾青素率高；事实上，它的细胞中含有80%以上的虾青素。这种显著的虾青素产量通常是在逆境条件下获得的，如营养缺乏（N或P）、高NaCl浓度、温度变化和其他因素。在此基础上，对雨芽虾青素的最佳生产条件进行了研究。本研究旨在分析光强、通气性和养分等环境因子对雨水杨生长和虾青素产生的影响。在28°c的BBM培养基中，连续白光照射(177 μ mol光子m(-2)s(-1))，连续曝气（1.5 v.v.m.），最大生长量为3.5 × 10(5)个/ml。在连续光照(345 μ mol光子m（-2) s(-1)）、乙酸钠浓度为1 g/l、不曝气的BAR培养基中，虾青素产量最高为98 mg/g生物量。(C) 2003 Elsevier Ltd.版权所有。
Astaxanthin extracted from green algae is desirable in the food and pharmaceutical industries due to its antioxidant properties. The green unicellular clear water microalga Haematococcus pluvialis has a high production rate of astaxanthin; indeed, it contains more than 80% astaxanthin content in its cells. This remarkable astaxanthin production is commonly obtained under stress conditions such as nutrient deficiency (N or P), high NaCl concentrations, variations of temperature, and other factors. In this vein, a great research effort has been oriented to determine optimal conditions for astaxanthin production by H. pluvialis. The objective of the present study was the analysis of environmental factors, such as light intensity, aeration and nutrients on the growth and astaxanthin production of H. pluvialis. Maximum growth of H. pluvialis obtained was 3.5 x 10(5) cells/ml in BBM medium at 28degreesC under continuous illumination (177 mumol photon m(-2)s(-1)) of white fluorescent light, with continuous aeration (1.5 v.v.m.). Meanwhile, maximal astaxanthin production was 98 mg/g biomass in BAR medium with continuous illumination (345 mumol photon m(-2) s(-1)), with l g/l of sodium acetate and without aeration. (C) 2003 Elsevier Ltd. All rights reserved.