The progression of peroxisomal degradation through autophagy requires peroxisomal division
The progression of peroxisomal degradation through autophagy requires peroxisomal division
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DOI：
10.4161/auto.27852
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发表时间：
2014-04-01
期刊：
影响因子：
13.3
通讯作者：
Klionsky, Daniel J.
中科院分区：
文献类型：
--
作者：
Mao, Kai;Liu, Xu;Klionsky, Daniel J.
过氧化物酶体是高度动态的细胞器，在细胞代谢中具有多种功能。为了使细胞内条件适应不断变化的细胞外环境，过氧化物酶体经历组成性分离和降解。过氧化物酶体的分离由2种动力蛋白相关的GTP酶Dnm 1和Vps1介导，而过氧化物酶体的降解是通过pexophagy（一种选择性自噬）完成的。在pexophagy，细胞器的大小总是一个具有挑战性的因素的吞噬效率的螯合室，吞噬细胞，这意味着过氧化物酶体分裂的降解过程中的潜在作用，类似的情况与选择性线粒体降解。在这项研究中，我们报告说，过氧化物酶体裂变确实是至关重要的有效消除的细胞器。当pexophagy被诱导时，Dnm1和Vps1都通过与Atg11和Atg36的相互作用被招募到降解过氧化物酶体中。此外，我们发现，特定的过氧化物酶体分裂，这是只需要pexophagy，发生在pexophagy的过氧化物酶体接触网站。
Peroxisomes are highly dynamic organelles that have multiple functions in cellular metabolism. To adapt the intracellular conditions to the changing extracellular environment, peroxisomes undergo constitutive segregation and degradation. The segregation of peroxisomes is mediated by 2 dynamin-related GTPases, Dnm1 and Vps1, whereas, the degradation of peroxisomes is accomplished through pexophagy, a selective type of autophagy. During pexophagy, the size of the organelle is always a challenging factor for the efficiency of engulfment by the sequestering compartment, the phagophore, which implies a potential role for peroxisomal fission in the degradation process, similar to the situation with selective mitochondria degradation. In this study, we report that peroxisomal fission is indeed critical for the efficient elimination of the organelle. When pexophagy is induced, both Dnm1 and Vps1 are recruited to the degrading peroxisomes through interactions with Atg11 and Atg36. In addition, we found that specific peroxisomal fission, which is only needed for pexophagy, occurs at mitochondria-peroxisome contact sites.