Adaptive responses to antimicrobial agents in biofilms.
Adaptive responses to antimicrobial agents in biofilms.
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DOI：
10.1111/j.1462-2920.2005.00797.x
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发表时间：
2005-08
影响因子：
5.1
通讯作者：
Barbara Szomolay;I. Klapper;J. Dockery;P. Stewart
中科院分区：
文献类型：
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作者：
Barbara Szomolay;I. Klapper;J. Dockery;P. Stewart
细菌生物膜比相应的浮游菌群更有效地表现出对抗菌应激的适应性耐药性。我们在此提出，在生物膜中，反应扩散有限的渗透可能导致生物膜更深区域的抗菌剂暴露水平较低。如果适应的局部时间尺度比消毒的时间尺度快，则受庇护的细胞能够进入适应的抵抗状态。这种机制不适用于浮游种群。提出一个数学模型来说明。结果表明，对于足够厚的生物膜，生物膜中的细胞比自由悬浮的细胞更有效地实施适应性反应。有效的消毒需要使用的杀菌剂浓度随着生物膜厚度呈二次或指数增加。
Bacterial biofilms demonstrate adaptive resistance in response to antimicrobial stress more effectively than corresponding planktonic populations. We propose here that, in biofilms, reaction-diffusion limited penetration may result in only low levels of antimicrobial exposure to deeper regions of the biofilm. Sheltered cells are then able to enter an adapted resistant state if the local time scale for adaptation is faster than that for disinfection. This mechanism is not available to a planktonic population. A mathematical model is presented to illustrate. Results indicate that, for a sufficiently thick biofilm, cells in the biofilm implement adaptive responses more effectively than do freely suspended cells. Effective disinfection requires applied biocide concentration that increases quadratically or exponentially with biofilm thickness.