NUCLEOLAR LOCALIZATION OF MYC TRANSCRIPTS
NUCLEOLAR LOCALIZATION OF MYC TRANSCRIPTS
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DOI：
10.1128/mcb.13.6.3221
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发表时间：
1993-06-01
影响因子：
5.3
通讯作者：
WOLD, B
中科院分区：
文献类型：
--
作者：
BOND, VC;WOLD, B
原位杂交显示c-myc RNA转录本在亚核内有明显的分布。有义链c-myc转录本的很大一部分定位于核仁。含有MYC内含子1的RNA明显不存在于核仁中，而是聚集在核质中。Myc RNA在核仁的定位在许多不同的细胞类型中是共同的，包括原代Sertoli细胞和几个细胞系。此外，核仁的定位并不局限于c-myc。N-myc和myoD转录本也表现出这种现象。相反，γ-肌动蛋白或乳酸脱氢酶转录本没有显示核仁定位。这些观察结果表明，核仁在潜在mRNAs的运输和/或周转中发挥了新的作用。
In situ hybridization has revealed a striking subnuclear distribution of c-myc RNA transcripts. A major fraction of the sense-strand nuclear c-myc transcripts was localized to the nucleoli. myc intron 1-containing RNAs were noticeably absent from nucleoli, accumulating instead in the nucleoplasm. The localization of myc RNA to nucleoli was shown to be common to a number of diverse cell types, including primary Sertoli cells and several cell lines. Furthermore, nucleolar localization was not restricted to c-myc. N-myc and myoD transcripts also displayed this phenomenon. In contrast, gamma-actin or lactate dehydrogenase transcripts did not display nucleolar localization. These observations suggest a new role for the nucleolus in transport and/or turnover of potential mRNAs.