BLOS1, a putative BLOC-1 subunit, interacts with SNX1 and modulates root growth in Arabidopsis
BLOS1, a putative BLOC-1 subunit, interacts with SNX1 and modulates root growth in Arabidopsis
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DOI：
10.1242/jcs.069732
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发表时间：
2010-11-01
影响因子：
4
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Cui, Yanyan;Li, Xugang;Li, Wei
大分子的内化和分选是所有真核细胞通过囊泡运输实现的固有特性。然而，这一过程对植物的了解相对较少。一个8亚基蛋白复合物，block -1，参与从核内体到溶酶体的内体运输，已经在人和小鼠中被鉴定出来。在这项研究中，该复合物的两个同源亚基，1（或AtGCN5L1）和2，已经在拟南芥中被表征。在分选内体上，1和2都与SNX1相互作用。低水平的可诱导RNAi系的主根较长，侧根较多。一致地，PIN1和PIN2在blon1 RNAi系中增加，暗示从核内体到液泡的运输受损。这些结果表明，在拟南芥中，一个已知的block -1复合物可能通过与SNX1的直接相互作用介导液泡降解运输，从而调节PIN1和PIN2的稳态，这对植物的生长发育至关重要。
Internalization and sorting of macromolecules are inherent properties of all eukaryotic cells that are achieved by vesicle trafficking. However, this process is relatively less understood in plants. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.