BLOOD COMPATIBILITY AND BIODEGRADABILITY OF PARTIALLY N-ACYLATED CHITOSAN DERIVATIVES
BLOOD COMPATIBILITY AND BIODEGRADABILITY OF PARTIALLY N-ACYLATED CHITOSAN DERIVATIVES
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DOI：
10.1016/0142-9612(95)98126-y
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发表时间：
1995-11-01
期刊：
影响因子：
14
通讯作者：
PARK, WH
中科院分区：
文献类型：
--
作者：
LEE, KY;HA, WS;PARK, WH
壳聚糖在甲醇的存在下选择性地与乙酸酐、丙酸酐、正丁酸酐、正戊酸酐和正己酸酐进行了n-酰化反应。采用0.3 ~ 1.2 mol /葡萄糖胺残基的羧酸酐，可得到20 ~ 50%的n -酰化程度，且不发生凝胶化。采用神学测定、凝血试验、扫描电镜观察等方法对n -酰基壳聚糖进行体外血液相容性试验。与其他方法相比，在考虑血液剪切流动效应的情况下，血凝蛋白神学测量结果准确、定量。n -酰基壳聚糖比n -乙酰壳聚糖具有更好的血液相容性，特别是n -己醇壳聚糖的血液相容性最好。通过将溶菌酶溶液加入到n -酰基壳聚糖溶液和膜中，在37℃下孵育，研究了酶降解过程。n -酰基壳聚糖对溶菌酶的敏感性与n -乙酰壳聚糖一样高。人们认为n -酰基壳聚糖的衍生化基团的数量和物理形态对其生物降解性有影响。溶菌酶作用下n -酰基壳聚糖膜释放的物质分子量为2 × 10(4) ~ 10 × 10(4)。
Chitosan was selectively N-acylated with various carboxylic anhydrides, e.g, acetic, propionic, n-butyric, n-valeric and n-hexanoic anhydrides, in the presence of methanol. The degree of N-acylation of about 20-50% was obtainable without occurrence of gelation by using carboxylic anhydrides of 0.3-1.2 mol per glucosamine residue. In vitro blood compatibility tests of N-acyl chitosans were performed by theological measurement, blood clotting test and scanning electron microscopic observation for human blood and plasma protein. The theological measurement of coagulation of plasma protein, considering the shear flow effect of blood, gave precise and quantitative results compared with other methods. N-Acyl chitosans showed more blood compatible properties than N-acety] chitosan and, in particular, N-hexanoyl chitosan was the most blood compatible. Enzymatic degradation was also investigated by adding a lysozyme solution to the N-acyl chitosan solution and film, incubating at 37 degrees C. N-Acyl chitosans had as high a susceptibility to lysozyme as N-acetyl chitosans. It was considered that the amount of derivatized groups and the physical form of N-acyl chitosans contributed to biodegradability. The molecular weight () of the material liberated from the N-acyl chitosan film by the action of lysozyme was 2 x 10(4)-10 x 10(4).