Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development from Cuttings of Ground-Cover Chrysanthemum and Associated Biochemical Changes
Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development from Cuttings of Ground-Cover Chrysanthemum and Associated Biochemical Changes
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DOI：
10.1007/s00344-010-9140-5
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Zhang, Mei-Ling
中科院分区：
文献类型：
--
作者：
Liao, Wei-Biao;Xiao, Hong-Lang;Zhang, Mei-Ling
外源一氧化氮（NO）和过氧化氢（H_2O_2）可以促进植物不定根的形成，但其生理机制尚不清楚。以地被菊花（Dendranthema morifolium 'Beiguozhicun'）为试材，研究了NO和H2O2对地被菊花扦插生根的影响及其生根过程中根区生化指标的变化。结果表明，NO和H2O2对地被菊扦插生根的影响具有剂量依赖性，NO供体硝普钠（SNP）浓度为50 μ M或H2O2浓度为200 μ M时，其生物学效应最大。NO和H_2O_2在诱导生根中具有协同效应。适宜剂量的NO和H2O2处理可提高地被菊插穗多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）的活性、水溶性碳水化合物（WSC）和总氮含量，降低总多酚含量。此外，生根率与这些生化成分的活性或含量呈显著相关。这些结果表明，NO和H2O2处理通过促进PPO和IAAO酶活性以及碳水化合物和氮的含量，同时抑制多酚的产生，协同和独立地促进不定根的发育。
It is well known that plant adventitious root formation can be stimulated by the application of nitric oxide (NO) and hydrogen peroxide (H2O2) exogenously but the mechanism of this physiological response is still unclear. Ground-cover chrysanthemum (Dendranthema morifolium 'Beiguozhicun') was used to understand the effects of NO and H2O2 on the rooting of plant cuttings and the associated biochemical changes of the rooting zone during the rhizogenesis process. The results showed that the effect of NO or H2O2 on rooting of ground-cover chrysanthemum cuttings was dose-dependent, with a maximal biological response at 50 mu M of NO donor sodium nitroprusside (SNP) or 200 mu M H2O2. There was a synergistic effect between NO and H2O2 on mediating rooting. NO and H2O2 treatments at the proper dosage might increase the activities of polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) and the content of water-soluble carbohydrate (WSC) and total nitrogen, while decreasing the total polyphenol content of ground-cover chrysanthemum cuttings. In addition, rooting percentage was significantly correlated with these biochemical constituent activities or contents. Together, these results indicated that NO and H2O2 treatments enhanced adventitious root development synergistically and independently by stimulating the activities of PPO and IAAO enzymes and the content of carbohydrate and nitrogen and simultaneously repressing the production of polyphenol.