High Oct4 expression: implications in the pathogenesis of neuroblastic tumours
High Oct4 expression: implications in the pathogenesis of neuroblastic tumours
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DOI：
10.1186/s12885-018-5219-3
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发表时间：
2019-01-03
期刊：
影响因子：
3.8
通讯作者：
Noguera, Rosa
中科院分区：
文献类型：
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作者：
Monferrer, Ezequiel;Burgos-Panadero, Rebeca;Noguera, Rosa
背景神经母细胞瘤（NBTs）是一种来源于胚胎神经嵴细胞的儿科实体瘤，这些神经嵴细胞含有自身的肿瘤干细胞（CSC）。有证据表明CSC可能是NBT中肿瘤进展、化疗耐药性和复发的原因。Oct 4是一种在哺乳动物胚胎发育和干细胞命运调控中起关键作用的转录因子。本研究的目的是阐明Oct 4在NBTs.MethodsWe的临床意义研究Oct 4表达在563原发NBTs使用数字图像定量。采用卡方检验分析组织病理学与Oct 4（+）细胞百分比的相关性。生存分析采用Kaplan-Meier曲线和log-rank检验。此外，采用逐步向后（Wald）方法进行多变量考克斯回归分析，以计算Oct 4表达水平对生存率的影响。结果我们发现，具有高比例表达Oct 4细胞的肿瘤与未分化和低分化的神经母细胞瘤/结节性神经节细胞瘤在统计学上相关，而Oct 4表达在神经节细胞瘤中不存在（P < 0.05）。
BackgroundNeuroblastic tumours (NBTs) are paediatric solid tumours derived from embryonic neural crest cells which harbour their own cancer stem cells (CSC). There is evidence indicating that CSC may be responsible for tumour progression, chemotherapy resistance and recurrence in NBTs. Oct4 is a transcription factor which plays a key role in mammal embryonic development and stem cell fate regulation. The aim of the study is to elucidate the clinical significance of Oct4 in NBTs.MethodsWe studied Oct4 expression in 563 primary NBTs using digital image quantification. Chi-square test was applied to analyse the correlation between histopathology and the Oct4(+) cell percentage. Survival analysis was carried out with Kaplan-Meier curves and log-rank test. Additionally, a multivariate Cox regression analysis with the stepwise backwards (Wald) method was undertaken to calculate the impact of Oct4 expression level on survival.ResultsWe found that tumours with a high proportion of cells expressing Oct4 correlated statistically with undifferentiated and poorly differentiated neuroblastoma / nodular ganglioneuroblastoma, and that Oct4 expression was not present in ganglioneuroma (p