The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism
The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism
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DOI：
10.1111/j.1365-2958.2006.05384.x
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发表时间：
2006-10-01
影响因子：
3.6
通讯作者：
Brantl, Sabine
中科院分区：
文献类型：
--
作者：
Heidrich, Nadja;Chinali, Alberto;Brantl, Sabine
虽然在大肠杆菌基因组中发现了大约70种小的非编码RNA，但对革兰氏阳性菌的调控RNA知之甚少。在这里，我们证明，最近确定的小的非翻译RNA SR 1从枯草芽孢杆菌基因组是一个监管RNA参与微调精氨酸catalysts。2D蛋白凝胶电泳显示了三个可能的SR 1靶点，它们受转录激活因子AhrC的调控，AhrC被证明是SR 1的主要靶点。体外配对研究和体内报告基因测试表明SR 1和ahrC mRNA之间存在特异性相互作用。这种相互作用并没有导致ahrC mRNA的降解，但在启动后阶段抑制翻译。我们的数据表明，Hfq分子伴侣是不需要的SR 1在体内的稳定。加入L-精氨酸和L-鸟氨酸后，SR 1的量增加，但L-瓜氨酸或L-脯氨酸则没有增加。
Whereas about 70 small non-coding RNAs have been found in the Escherichia coli genome, relatively little is known about regulatory RNAs from Gram-positive bacteria. Here, we demonstrate that the recently identified small untranslated RNA SR1 from the Bacillus subtilis genome is a regulatory RNA involved in fine-tuning of arginine catabolism. 2D protein gel electrophoresis indicated three possible SR1 targets that are regulated by the transcriptional activator AhrC, which was shown to be the primary target of SR1. In vitro pairing studies and an in vivo reporter gene test demonstrated a specific interaction between SR1 and ahrC mRNA. This interaction did not lead to degradation of ahrC mRNA, but inhibited translation at a post-initiation stage. Our data show that the Hfq chaperone was not required for the stabilization of SR1 in vivo. The amount of SR1 was increased upon addition of L-arginine and L-ornithine, but not L-citrulline or L-proline.