Genomic organization of the X-linked inhibitor of apoptosis and identification of a novel testis-specific transcript
Genomic organization of the X-linked inhibitor of apoptosis and identification of a novel testis-specific transcript
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DOI：
10.1006/geno.2001.6635
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发表时间：
2001-10-01
期刊：
影响因子：
4.4
通讯作者：
Korneluk, RG
中科院分区：
文献类型：
--
作者：
Lagacé, M;Xuan, JY;Korneluk, RG
在这里，我们报告的X-连锁的细胞凋亡抑制基因（BIRC 4，也被称为XIAP和hILP）的基因组组织和映射和一个密切相关的转录本的鉴定。BIRC 4位于Xq 25上，由7个外显子组成。内含子/外显子结构在小鼠同源物和其人类对应物之间是高度保守的。在基因组Southern印迹上，四条带与BIRC 4编码区探针交叉反应。这些交叉反应条带之一编码一个无内含子基因，该基因仅在睾丸中表达2.2 kb的转录本。该睾丸特异性转录物含有与BIRC 4的羧基末端同源的推定开放阅读框（ORF）;该ORF的过表达显示对BAX诱导的细胞凋亡的保护作用。
Here we report the genomic organization and mapping of the X-linked inhibitor of apoptosis gene (BIRC4, also known as XIAP and hILP) and the identification of a closely related transcript. BIRC4 is located on Xq25 and is composed of seven exons. The intron/exon structure is highly conserved between the mouse homologue and its human counterpart. Four bands cross-react with a BIRC4 coding region probe on a genomic Southern blot. One of these cross-reactive bands encodes an intronless gene that expresses a 2.2-kb transcript solely in the testis. This testis-specific transcript contains a putative open reading frame (ORF) that is homologous to the carboxy-terminal end of BIRC4; overexpression of this ORF shows protective effects against BAX-induced apoptosis.