Insulator-based dielectrophoresis of microorganisms: Theoretical and experimental results
Insulator-based dielectrophoresis of microorganisms: Theoretical and experimental results
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DOI：
10.1002/elps.201100168
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发表时间：
2011-09-01
期刊：
影响因子：
2.9
通讯作者：
Lapizco-Encinas, Blanca H.
中科院分区：
文献类型：
--
作者：
Moncada-Hernandez, Hector;Baylon-Cardiel, Javier L.;Lapizco-Encinas, Blanca H.
介电电泳（DEP）是粒子在非均匀电场中由于极化效应而产生的运动。DEP具有处理细胞的巨大潜力，并且是一种非破坏性现象。它已被用于不同的细胞分析，从活力评估到浓缩富集和分离。基于绝缘体的DEP（iDEP）提供了一个有吸引力的替代传统的基于电极的系统;在iDEP中，绝缘结构用于产生不均匀的电场，从而产生更简单，更强大的设备。尽管用于细胞应用的iDEP微器件发展迅速，但细胞介电电泳行为背后的基本原理尚未完全阐明。理解iDEP背后的理论对于继续这一领域的进展是必要的。本工作介绍了使用iDEP操作和分离细菌和酵母细胞。采用COMSOL Multiphysics中的计算模型预测直流iDEP对悬浮在包含绝缘结构阵列的微通道中的细胞的影响。该模型允许预测粒子的行为，pathlines和介电电泳固定应该发生的区域。实验工作进行了相同的操作条件下采用的模型和结果进行了比较，获得良好的协议。这是第一次报告的数学建模的酵母和细菌细胞的介电电泳响应的DC-iDEP微器件。
Dielectrophoresis (DEP) is the motion of particles due to polarization effects in nonuniform electric fields. DEP has great potential for handling cells and is a non-destructive phenomenon. It has been utilized for different cell analysis, from viability assessments to concentration enrichment and separation. Insulator-based DEP (iDEP) provides an attractive alternative to conventional electrode-based systems; in iDEP, insulating structures are used to generate nonuniform electric fields, resulting in simpler and more robust devices. Despite the rapid development of iDEP microdevices for applications with cells, the fundamentals behind the dielectrophoretic behavior of cells has not been fully elucidated. Understanding the theory behind iDEP is necessary to continue the progress in this field. This work presents the manipulation and separation of bacterial and yeast cells with iDEP. A computational model in COMSOL Multiphysics was employed to predict the effect of direct current-iDEP on cells suspended in a microchannel containing an array of insulating structures. The model allowed predicting particle behavior, pathlines and the regions where dielectrophoretic immobilization should occur. Experimental work was performed at the same operating conditions employed with the model and results were compared, obtaining good agreement. This is the first report on the mathematical modeling of the dielectrophoretic response of yeast and bacterial cells in a DC-iDEP microdevice.