In vitro interaction of Mycobacterium avium with intestinal epithelial cells
In vitro interaction of Mycobacterium avium with intestinal epithelial cells
复制标题
鸟分枝杆菌与肠上皮细胞的体外相互作用
作者：
M. E. Mapother;J. Songer
用鸟分枝杆菌血清型 2、8 或 10 的培养物接种人肠上皮细胞单层，这些培养物是活的、高压灭菌、福尔马林灭活、暴露于紫外线或悬浮于抗分枝杆菌中。 avium 血清型 2 血清。还研究了四种已知可阻断吞噬作用或内吞作用的试剂（细胞松弛素 B、二丁酰环单磷酸腺苷、碘乙酸盐和 2,4-二硝基苯酚）对细菌-细胞相互作用的影响。人类肠上皮细胞对致病性鸟支原体的最大摄取发生在孵育 2 至 3 小时后。血清型 2 的吸收量高于血清型 8 或 10。腐生分枝杆菌不附着或渗透宿主细胞。数据显示，存活的分枝杆菌会被宿主细胞摄入，而死亡的生物体则不会。细菌细胞的成分部分但不是唯一地负责人肠上皮细胞对鸟分枝杆菌血清型 2 的吞噬。此外，人肠上皮细胞对鸟分枝杆菌的摄取被抑制已知吞噬细胞摄取的试剂所抑制，表明摄取机制是由有毒分枝杆菌诱导的内吞过程。
Human intestinal epithelial cell monolayers were inoculated with cultures of Mycobacterium avium serotype 2, 8, or 10 that were viable, autoclaved, Formalin killed, exposed to UV light, or suspended in anti-M. avium serotype 2 serum. The effects of four reagents known to block phagocytosis or endocytosis (cytochalasin B, dibutyryl cyclic adenosine monophosphate, iodoacetate, and 2,4-dinitrophenol) on the bacteria-cell interaction were also studied. The maximum uptake of pathogenic M. avium by human intestinal epithelial cells occurred after 2 to 3 h of incubation. Serotype 2 was taken up in greater quantity than serotype 8 or 10. Saprophytic mycobacteria did not attach to or penetrate the host cells. The data showed that viable mycobacteria are ingested by host cells, whereas dead organisms are not. Components of the bacterial cells are partially, but not solely, responsible for the phagocytosis of M. avium serotype 2 by human intestinal epithelial cells. Furthermore, uptake of M. avium by human intestinal epithelial cells was suppressed by reagents which inhibit uptake by known phagocytic cells, suggesting that the mechanism of uptake is an endocytic process induced by virulent mycobacteria.