Systematic Analysis of the DNA Methylase and Demethylase Gene Families in Rapeseed (Brassica napus L.) and Their Expression Variations After Salt and Heat stresses
Systematic Analysis of the DNA Methylase and Demethylase Gene Families in Rapeseed (Brassica napus L.) and Their Expression Variations After Salt and Heat stresses
复制标题
油菜 DNA 甲基化酶和去甲基化酶基因家族的系统分析及其在盐和热胁迫后的表达变化
DOI：
10.3390/ijms21030953
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Fan, Shihang;Liu, Hongfang;Li, Jun
DNA甲基化是将甲基添加到DNA分子中的过程，从而在不改变序列的情况下改变DNA片段的活性。越来越多的证据表明，DNA甲基化通过基因组印记和转座因子抑制等关键过程参与植物生长发育的各个方面。DNA甲基化酶和去甲基化酶是维持植物基因组DNA甲基化状态的两个关键酶。本研究在油菜（Brassica napus L.）中鉴定了22个DNA甲基化酶基因和6个DNA去甲基化酶基因。基因组这些DNA甲基化酶和DNA去甲基化酶基因可分别分为4个亚家族（BnaCMT、BnaMET 1 s、BnaDRM和BnaDNMT 2s）和3个亚家族（BnaDME、BnaDML 3和BnaROS 1 s）。基因结构和保守结构域的分析表明，油菜和拟南芥中的每个亚类都是高度保守的。通过RNA-seq和qRT-PCR进行的表达分析表明，这些DNA甲基化/去甲基化相关基因可能参与了油菜的热/盐胁迫反应。两者合计，我们的研究结果可能提供有价值的信息，为未来的功能特性，这两种类型的表观遗传调控酶在多倍体物种，如油菜籽，以及分析它们在植物界的进化关系。
DNA methylation is a process through which methyl groups are added to the DNA molecule, thereby modifying the activity of a DNA segment without changing the sequence. Increasing evidence has shown that DNA methylation is involved in various aspects of plant growth and development via a number of key processes including genomic imprinting and repression of transposable elements. DNA methylase and demethylase are two crucial enzymes that play significant roles in dynamically maintaining genome DNA methylation status in plants. In this work, 22 DNA methylase genes and six DNA demethylase genes were identified in rapeseed (Brassica napus L.) genome. These DNA methylase and DNA demethylase genes can be classified into four (BnaCMTs, BnaMET1s, BnaDRMs and BnaDNMT2s) and three (BnaDMEs, BnaDML3s and BnaROS1s) subfamilies, respectively. Further analysis of gene structure and conserved domains showed that each sub-class is highly conserved between rapeseed and Arabidopsis. Expression analysis conducted by RNA-seq as well as qRT-PCR suggested that these DNA methylation/demethylation-related genes may be involved in the heat/salt stress responses in rapeseed. Taken together, our findings may provide valuable information for future functional characterization of these two types of epigenetic regulatory enzymes in polyploid species such as rapeseed, as well as for analyzing their evolutionary relationships within the plant kingdom.